UASp regulatory sequences drive expression of sra tagged at the C-terminal end with 3xTag:FLAG. The serine at residue 72 has been replaced by alanine.
Scer\GAL4VP16.nanos.UTR/sraS72A.UASp.Tag:FLAG rescues sraKO
Expression of sraS72A.Scer\UAS.P\T.T:Zzzz\FLAG under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the meiotic phenotype seen in sraKO mutants. 97.6% of eggs are able to complete meiosis.