FlyBase Allele Report: Dmel\sra[KO]

General Information

Symbol

Dmel\sra^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0194818

Feature type

allele

Associated gene

Dmel\sra

Associated Insertion(s)

TI{TI}sra^KO

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Takeo et al., 2006)

Mutagen

recombination

(Takeo et al., 2006)

FLPase

(Takeo et al., 2006)

I-SceI

(Takeo et al., 2006)

gene targeting by homologous recombination

(Takeo et al., 2006)

Progenitor genotype

Associated Insertion(s)

TI{TI}sra^KO

Cytology

Description

Part of the sra gene has been replaced by a w^+* marker.

(Takeo et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg, with Scer\GAL4^{VP16.nanos.UTR}, sra^S215A.UASp

(Takeo et al., 2010)

egg, with Scer\GAL4^{VP16.nanos.UTR}, sra^S219A.UASp

(Takeo et al., 2010)

egg & meiotic anaphase I

(Takeo et al., 2006)

egg & meiotic anaphase I | germ-line clone

(Takeo et al., 2006)

eye

(Lee et al., 2016)

Detailed Description

Statement

Reference

sra^KO heterozygote adults display mild rough-eye phenotype compared to controls.

(Lee et al., 2016)

sra^KO flies move throughout a 24 hour period and are approximately twofold more active than wild-type flies in both LD and DD conditions. Heterozygous sra^KO mutants have identical activity to wild-type flies.

Compared to wild-type flies, sra^KO flies show a dramatic loss of sleep, to approximately 30% of wild-type amounts. This result is also observed in DD conditions.

Walking activity is slightly decreased in LD conditions in sra^KO mutants, while it is only slightly increased in DD conditions. The increase in daily activity of sra^KO mutants is due to increased time spent in a walking state, rather than to increased activity during walking.

The number of daily sleep bouts is the same in sra^KO mutants and wild-type flies, in both LD and DD conditions. In contrast, the average sleep bout length in sra^KO mutants is decreased to approximately 30% that of wild-type, in LD and DD conditions, indicating that decreased sleep in sra^KO is due to a decrease in sleep maintenance, rather than to a decrease in sleep onset.

sra^GS3080/sra^KO mutants exhibit a precipitous decrease in time spent asleep, approximately one third of the amount of wild-type sleep.

sra^KO flies tend to die much earlier than wild-type flies.

(Nakai et al., 2011)

The defective egg hatching phenotype resulting from the mis-expression of sra^{S215A.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} is strongly enhanced by sra^KO heterozygosity.

The rate of egg hatching appears normal as a result of sra^{S219A.Scer\UAS.P\T} expression driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16}. The rate of egg hatching is reduced by sra^KO heterozygosity.

(Takeo et al., 2010)

Approximately 70% of sra^KO individuals develop to the adult stage, but these adults have shorter lives than control flies. Virgin females show spontaneous ovulation.

(Takeo et al., 2006)

The ovaries of sra^KO females appear morphologically normal but eggs from sra^KO mothers arrest in anaphase I of meiosis I and fail to hatch. Eggs from sra^KO germline clones also arrest at anaphase I.

(Takeo et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

sra^GS3080/sra^KO has abnormal sleep phenotype, suppressible | partially by CanA-14F^KO

(Nakai et al., 2011)

sra^GS3080/sra^KO has abnormal sleep phenotype, suppressible | partially by Pp2B-14D^KO

(Nakai et al., 2011)

Enhancer of

Statement

Reference

sra^GS3080/sra^KO is an enhancer of abnormal sleep phenotype of CanA-14F^KO

(Nakai et al., 2011)

sra^GS3080/sra^KO is an enhancer of abnormal sleep phenotype of Pp2B-14D^KO

(Nakai et al., 2011)

sra[+]/sra^KO is an enhancer of partially lethal - majority die | germline clone phenotype of Pp2B-14D^DN.UASp, Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2010)

Suppressor of

Statement

Reference

sra^KO is a suppressor of visible | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Other

Statement

Reference

Pp2B-14D^DN.UASp, Scer\GAL4^{VP16.nanos.UTR}, sra[+]/sra^KO has abnormal meiotic cell cycle phenotype

(Takeo et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

sra^KO is a suppressor of eye phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Other

Statement

Reference

Pp2B-14D^DN.UASp, Scer\GAL4^{VP16.nanos.UTR}, sra[+]/sra^KO has polar body nucleus phenotype

(Takeo et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The severe small and rough eye phenotype observed in flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^GMR.PU is significantly ameliorated by combination with sra^KO in heterozygous state.

(Lee et al., 2016)

A CanA-14F^KO background partially suppresses the reduction in total sleep found in sra^GS3080/sra^KO mutants.

A Pp2B-14D^KO background partially suppresses the reduction in total sleep found in sra^GS3080/sra^KO mutants.

(Nakai et al., 2011)

Heterozygosity for sra^KO enhances the lethality resulting from the overexpression of Pp2B-14D^{DN.Scer\UAS.P\T} in the female germ-line driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16}. Most of the mutant cells fail to complete meiosis. Defects in these eggs vary from oocyte to oocyte, but a large number of eggs lack a characteristic structure of polar bodies known as the rosette structure formed after two rounds of meiotic division. This strong genetic interaction is not observed by overexpression of Pp2B-14D^{Scer\UAS.P\T.cTa}.

(Takeo et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sra^KO is rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^{UASp.Tag:FLAG}

(Takeo et al., 2012)

sra^KO is rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^{S67A.UASp.Tag:FLAG}

(Takeo et al., 2012)

sra^KO is rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^{S72A.UASp.Tag:FLAG}

(Takeo et al., 2012)

sra^KO is rescued by sra^{S100A.UASp.Tag:FLAG}/Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2012)

sra^KO is rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^{T102A.UASp.Tag:FLAG}

(Takeo et al., 2012)

sra^KO is rescued by sra^{T196A.UASp.Tag:FLAG}/Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2012)

sra^KO is rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^{T246A.UASp.Tag:FLAG}

(Takeo et al., 2012)

sra^KO is rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^{S199A.UASp.Tag:FLAG}

(Takeo et al., 2012)

sra^KO is rescued by sra^UAS.cEa/Scer\GAL4^elav.PLu

(Nakai et al., 2011)

Partially rescued by

sra^KO is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^{S219A.UASp.Tag:FLAG}

(Takeo et al., 2012)

sra^KO is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^{S219D.UASp.Tag:FLAG}

(Takeo et al., 2012)

sra^KO is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^{S219E.UASp.Tag:FLAG}

(Takeo et al., 2012)

sra^KO is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^S219A.UASp

(Takeo et al., 2010)

sra^KO is partially rescued by sra^hs.PT

(Takeo et al., 2006)

sra^KO is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^UASp.cTa

(Takeo et al., 2006)

Not rescued by

sra^KO is not rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^{S215A.UASp.Tag:FLAG}

(Takeo et al., 2012)

sra^KO is not rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^{S215D.UASp.Tag:FLAG}

(Takeo et al., 2012)

sra^KO is not rescued by sra^{S215E.UASp.Tag:FLAG}/Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2012)

sra^KO is not rescued by sra^S215A.UASp/Scer\GAL4^{VP16.nanos.UTR}

(Takeo et al., 2010)

sra^KO is not rescued by Scer\GAL4^{VP16.nanos.UTR}/sra^{Δ212-221.UASp}

(Takeo et al., 2010)

Comments

Expression of sra^{Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the female sterility and meiotic phenotype seen in sra^KO mutants. 95.6% of the laid eggs are able to complete meiosis.

Expression of sra^{S67A.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the meiotic phenotype seen in sra^KO mutants. 87.9% of eggs are able to complete meiosis.

Expression of sra^{S72A.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the meiotic phenotype seen in sra^KO mutants. 97.6% of eggs are able to complete meiosis.

Expression of sra^{S100A.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the meiotic phenotype seen in sra^KO mutants. All eggs are able to complete meiosis.

Expression of sra^{T102A.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the meiotic phenotype seen in sra^KO mutants. All eggs are able to complete meiosis.

Expression of sra^{T196A.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the meiotic phenotype seen in sra^KO mutants. 97.1% of eggs are able to complete meiosis.

Expression of sra^{S215A.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to rescue the meiotic phenotype seen in sra^KO mutants. No eggs are able to complete meiosis. Expressing sra^{S215A.Scer\UAS.P\T.T:Zzzz\FLAG} in a sra^KO/+ background results in only 8% of eggs hatching and meiotic defects are observed.

Expression of sra^{S219A.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} partially rescues the meiotic phenotype seen in sra^KO mutants. Only 26.2% of eggs are able to complete meiosis.

Expression of sra^{T246A.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the meiotic phenotype seen in sra^KO mutants. 93.2% of eggs are able to complete meiosis.

Expression of sra^{S199A.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the meiotic phenotype seen in sra^KO mutants. 86.2% of eggs are able to complete meiosis.

Expression of sra^{S215D.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to rescue the meiotic phenotype seen in sra^KO mutants. No eggs are able to complete meiosis.

Expression of sra^{S215E.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to rescue the meiotic phenotype seen in sra^KO mutants. No eggs are able to complete meiosis.

Expression of sra^{S219D.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} partially rescues the meiotic phenotype seen in sra^KO mutants. 60.0% of eggs are able to complete meiosis.

Expression of sra^{S219E.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} partially rescues the meiotic phenotype seen in sra^KO mutants. 71.9% of eggs are able to complete meiosis.

(Takeo et al., 2012)

Expression of sra^Scer\UAS.cEa in the nervous system under the control of Scer\GAL4^elav.PLu restores the increased locomotor activity found in sra^KO mutants back to wild-type levels. Similarly, sleep is completely restored to wild-type amounts in this genotype. Concurrent with this, a wild-type lifespan is also restored.

(Nakai et al., 2011)

sra^{S215A.Scer\UAS.P\T} driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to restore female fertility or meiotic phenotype in sra^KO homozygotes.

sra^{Δ212-221.Scer\UAS.P\T} driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to restore female fertility or meiotic phenotype in sra^KO homozygotes.

sra^{S219A.Scer\UAS.P\T} driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} partially restores female fertility or meiotic phenotype in sra^KO homozygotes. Meiosis is completed completed and synchronous mitotic division is observed in about 11% of the mutant eggs. The remaining eggs exhibit multiple aster-free spindle phenotypes.

(Takeo et al., 2010)

The sra^{Scer\UAS.P\T.cTa} transgene, when driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16}, is able to rescue the semilethality and the spontaneous ovulation phenotypes of sra^KO females, but can only partially rescue the female sterility.

The sra^hs.PT transgene is not able to rescue the female sterility of sra^KO flies.

(Takeo et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

607149

w^*; TI{TI}sra^KO/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

sra^KO

(Lee et al., 2016, Takeo et al., 2012, Nakai et al., 2011, Takeo et al., 2010, Takeo et al., 2006, Takeo et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (6)

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