UAS regulatory sequences drive expression of a DsRed(Unk) fluorescent protein. A genomic fragment containing the mir-303 pre-miRNA hairpin has been inserted into the DsRed(Unk) 3'UTR.
Expression via Scer\GAL4Bx-MS1096 results in lethality/extra macrochaetes in notum and small, curled, folded wings.
Expression via Scer\GAL4GMR.PU results in rough eyes
Expression via Scer\GAL4ptc.PU results in curled wings and big halteres.
Expression via Scer\GAL4Eq results in misorganized microchaetes.
Expression via Scer\GAL4sca.PU results in short/thin macrochaetes.
Expression via Scer\GAL4ey.PU results in lethality and a mild eye phenotype.
Expression via Scer\GAL4sd.PU results in an irregular wing margin.
Expression of mir-303Scer\UAS.T:Disc\RFP under the control of Scer\GAL4Bx-MS1096 results in male lethality. Surviving females have wings which are usually very small, folded and have strong defects in patterning.
Expression of mir-303Scer\UAS.T:Disc\RFP under the control of Scer\GAL4sd-SG29.1 results in small folded wings which may have an irregular wing margin.
Expression of mir-303Scer\UAS.T:Disc\RFP under the control of Scer\GAL4ptc-559.1 results in a curved wing.