UAS regulatory sequences drive expression of a DsRed(Unk) fluorescent protein. A genomic fragment containing an miRNA cluster (includes the mir-984, mir-983-1 and mir-983-2 pre-miRNA hairpins) has been inserted into the DsRed(Unk) 3'UTR.
Expression of P{UAS-DsRed-mir-984+mir-983-1+mir-983-2.L} via Scer\GAL4Bx-MS1096 results in lethality with small, curled, folded, blistered wings.
Expression of P{UAS-DsRed-mir-984+mir-983-1+mir-983-2.L} via Scer\GAL4GMR.PU results in rough, big eyes.
Expression of P{UAS-DsRed-mir-984+mir-983-1+mir-983-2.L} via Scer\GAL4ptc.PU results in lethality or a quick death. Macrochaetes are missing, scutellum is short.
Expression of P{UAS-DsRed-mir-984+mir-983-1+mir-983-2.L} via Scer\GAL4Eq results in misorganized or missing microchaetes.
Expression of P{UAS-DsRed-mir-984+mir-983-1+mir-983-2.L} via Scer\GAL4sca.PU results in lethality and missing macrochaetes.
Expression of P{UAS-DsRed-mir-984+mir-983-1+mir-983-2.L} via Scer\GAL4ey.PU is lethal with a misformed head.
Expression of P{UAS-DsRed-mir-984+mir-983-1+mir-983-2.L} via Scer\GAL4sd.PU is lethal with no wings.
Expression of P{UAS-DsRed-mir-984+mir-983-1+mir-983-2.L} under the control of Scer\GAL4Bx-MS1096 results in male lethality. Females have very short wings which are curved and folded and have blisters covering the entire wing.
Adult escapers expressing P{UAS-DsRed-mir-984+mir-983-1+mir-983-2.L} under the control of Scer\GAL4sd-SG29.1 lack wings.