FlyBase Allele Report: Dmel\stck[Q38A.Tag:FLAG]

General Information

Symbol

Dmel\stck^{Q38A.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0278274

Feature type

allele

Associated gene

Dmel\stck

Associated Insertion(s)

Carried in Construct

P{stck-FLAG.Q38A}

Also Known As

PINCH^Q38A-Flag

Key Links

Transgenic product class

missense_variant

(Elias et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Elias et al., 2012)

Mutagen

in vitro construct

(Elias et al., 2012)

Progenitor genotype

Carried in construct

P{stck-FLAG.Q38A}

Cytology

Description

The endogenous stck promoter drives expression of a mutant form of stck in which the glutamine at amino acid 38 has been replaced by alanine. The construct is tagged at the C-terminal end with Tag:FLAG.

(Elias et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

stck

Encoded product / tool

stck

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of stck^{Q38A.T:Zzzz\FLAG} in an otherwise wild-type background has no effect on larval size.

(Pronovost et al., 2013)

No obvious phenotypes are seen when stck^{Q38A.T:Zzzz\FLAG} is expressed in wild type flies.

Embryos expressing stck^{Q38A.T:Zzzz\FLAG} in a stck^3R-17/stck¹⁸ mutant background do not display any muscle structure or actin cytoskeleton defects.<D-c>

(Elias et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

stck^{Q38A.Tag:FLAG} is an enhancer of lethal phenotype of flw^G0172

(Pronovost et al., 2013)

stck^{Q38A.Tag:FLAG} is an enhancer of decreased body size | larval stage phenotype of flw^G0172

(Pronovost et al., 2013)

stck^{Q38A.Tag:FLAG} is an enhancer of abnormal locomotor behavior | larval stage phenotype of flw^G0172

(Pronovost et al., 2013)

Other

Statement

Reference

flw^G0172, stck^{Q38A.Tag:FLAG} has lethal - all die before end of larval stage phenotype

(Pronovost et al., 2013)

ics^BG02577, stck¹⁸/stck^3R-17, stck^{Q38A.Tag:FLAG} has partially lethal - majority die phenotype

(Elias et al., 2012)

ics^BG02577, stck¹⁸/stck^3R-17, stck^{Q38A.Tag:FLAG} has abnormal developmental rate | first instar larval stage phenotype

(Elias et al., 2012)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

stck^{Q38A.Tag:FLAG} is a non-suppressor of larval thoracic ventral intersegmental muscle cell | larval stage phenotype of flw^G0172

(Pronovost et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

flw^G0172 mutants carrying stck^{Q38A.T:Zzzz\FLAG} arrest during the larval stages. The larvae are significantly smaller and significantly less motile than flw^G0172 single mutant larvae. Expression of stck^{Q38A.T:Zzzz\FLAG} does not rescue the detachment of ventral intersegmental muscles seen in flw^G0172 larvae.

(Pronovost et al., 2013)

The addition of ics^BG02577 reduces the viability of flies expressing stck^{Q38A.T:Zzzz\FLAG} in a stck^3R-17/stck¹⁸ mutant background. Between 6 and 34% of the expected number of progeny are seen, with the majority of the lethality taking place during the larval and pharate stages. The rescued larvae display a range of sizes, including presumptive first instar larvae that appear stumpy, exhibit sluggish movement and do not progress in development.

(Elias et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

stck^{Q38A.Tag:FLAG} rescues stck¹⁸/stck^3R-17

(Elias et al., 2012)

Comments

Expression of stck^{Q38A.T:Zzzz\FLAG} rescues the embryonic and larval lethality seen in stck^3R-17/stck¹⁸ mutants. The rescued flies are fertile and do not display either wing blisters or any other overt phenotypes.

(Elias et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66576

w^*; P{stck-FLAG.Q38A}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

stck^{Q38A.T:Zzzz\FLAG}

stck^{Q38A.Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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