FlyBase Allele Report: Dmel\Marf[UAS.cDa]

General Information

Symbol

Dmel\Marf^UAS.cDa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of DuBoff

FlyBase ID

FBal0278276

Feature type

allele

Associated gene

Dmel\Marf

Associated Insertion(s)

Carried in Construct

P{UAS-Marf.D}

Also Known As

UAS-MARF

Key Links

Transgenic product class

cDNA

(Duboff et al., 2012)

wild_type

(Duboff et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Duboff et al., 2012)

wild_type

(Duboff et al., 2012)

Mutagen

in vitro construct

(Duboff et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Marf.D}

Cytology

Description

UASt regulatory sequences drive expression of a Marf cDNA (RE04414).

(Duboff et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Marf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Duboff et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death, with Scer\GAL4^elav.PU

(Duboff et al., 2012)

abnormal neuroanatomy, with Scer\GAL4^elav.PU

(Duboff et al., 2012)

Phenotype Manifest In

adult brain, with Scer\GAL4^elav.PU

(Duboff et al., 2012)

mitochondrion, with Scer\GAL4^elav.PU

(Duboff et al., 2012)

Detailed Description

Statement

Reference

Expression of Marf^Scer\UAS.cDa under the control of Scer\GAL4^elav.PU results in an increase in mitochondria length in adult neurons. A small increase in neurodegeneration (assayed using TUNEL) is also observed, as is an increase in superoxide production (assayed using dihydroethidium). Levels of cell cycle activation (assayed by PCNA) are similar to controls.

(Duboff et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Marf^UAS.cDa, Scer\GAL4^elav.PU has abnormal neuroanatomy phenotype, enhanceable by Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Marf^UAS.cDa, Scer\GAL4^elav.PU has abnormal cell death phenotype, enhanceable by Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Enhancer of

Statement

Reference

Marf^UAS.cDa, Scer\GAL4^elav.PU is an enhancer of abnormal neuroanatomy phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Marf^UAS.cDa, Scer\GAL4^elav.PU is an enhancer of abnormal cell death phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Marf^UAS.cDa, Scer\GAL4^elav.PU is an enhancer of abnormal cell cycle phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Marf^UAS.cDa, Scer\GAL4^elav.PU has adult brain phenotype, enhanceable by Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Marf^UAS.cDa, Scer\GAL4^elav.PU has mitochondrion phenotype, enhanceable by Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Enhancer of

Statement

Reference

Marf^UAS.cDa, Scer\GAL4^elav.PU is an enhancer of adult brain phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Marf^UAS.cDa, Scer\GAL4^elav.PU is an enhancer of mitochondrion phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Suppressor of

Statement

Reference

Marf^UAS.cDa, Scer\GAL4^Bx-MS1096 is a suppressor of wing | male limited phenotype of Scer\GAL4^Bx-MS1096, fog^UAS.cRa

(Ratnaparkhi, 2013)

Marf^UAS.cDa, Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, fog^UAS.cRa

(Ratnaparkhi, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Marf^Scer\UAS.cDa under the control of Scer\GAL4^Bx-MS1096 suppresses the wing phenotype found upon expression of fog^Scer\UAS.cRa : in >80% of F1 males, the wings appear normal, whereas the F1 females do not show any wing defects.

(Ratnaparkhi, 2013)

Xenogenetic Interactions

Statement

Reference

The increase in mitochondria length seen when either Marf^Scer\UAS.cDa or Hsap\MAPT^{R406W.Scer\UAS} are expressed under the control of Scer\GAL4^elav.PU is enhanced when the two constructs are co-expressed. Increased levels of neurodegeneration and cell cycle activation are also seen.

Expression of Marf^Scer\UAS.cDa enhances the increase in superoxide production seen when Hsap\MAPT^{R406W.Scer\UAS} is expressed under the control of Scer\GAL4^elav.PU.

(Duboff et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Marf^Scer\UAS.cDa

Marf^UAS.cDa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of DuBoff

Secondary FlyBase IDs

References (2)

Report Sections

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