FlyBase Allele Report: Dmel\mir-34[null]

General Information

Symbol

Dmel\mir-34^null

Species

D. melanogaster

Name

FlyBase ID

FBal0278292

Feature type

allele

Associated gene

Dmel\mir-34

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Liu et al., 2012)

Progenitor genotype

Caused by aberration

Df(3R)mir-34

Cytology

Description

Deficiency breakpoint within the mir-34 locus.

(Liu et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain | conditional, with Fmr1^+tLa, mir-277^+tLa

(Liu et al., 2012)

adult gut

(Sreejith et al., 2025)

adult gut | chemical conditional

(Sreejith et al., 2025)

enteroblast | decreased number

(Sreejith et al., 2025)

indirect flight muscle cell

(Fulga et al., 2015)

intestinal stem cell | increased number

(Sreejith et al., 2025)

Detailed Description

Statement

Reference

mir-34^null animals display a progressive, age-dependent loss of flight resulting in a penetrant flightless phenotype at 30 days post eclosion. Indirect flight muscle myofibril structure shows marked defects in integrity and sarcomere organization.

(Fulga et al., 2015)

mir-34^null mutants (expressing mir-277^+tLa and Fmr1^+tLa to restore mir-277 and Fmr1 function respectively) do not display any obvious developmental defects. However, these flies show a catastrophic decline in viability just after 30 days. Three day old mutants display normal locomotion and stress resistance, but by 20 days the mutants have dramatic climbing defects and are markedly more sensitive to heat stress compared to age-matched controls.

The brains of mir-34^null mutants (expressing mir-277^+tLa and Fmr1^+tLa to restore mir-277 and Fmr1 function respectively) show dramatic vacuolisation with age, in contrast to the sporadic vacuoles seen in the brains of age-matched controls. An increased number of inclusions is also seen at 30 days compared to controls.

(Liu et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Fmr1^+tLa, mir-277^+tLa, mir-34^null has short lived phenotype, suppressible | partially by Eip74EF^BG01805

(Liu et al., 2012)

Fmr1^+tLa, mir-277^+tLa, mir-34^null has abnormal neuroanatomy | conditional phenotype, suppressible | partially by Eip74EF^BG01805

(Liu et al., 2012)

NOT suppressed by

Statement

Reference

Fmr1^+tLa, mir-277^+tLa, mir-34^null has short lived phenotype, non-suppressible by Eip74EF[+]/Eip74EF^BG01805

(Liu et al., 2012)

Fmr1^+tLa, mir-277^+tLa, mir-34^null has abnormal neuroanatomy | conditional phenotype, non-suppressible by Eip74EF[+]/Eip74EF^BG01805

(Liu et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Fmr1^+tLa, mir-277^+tLa, mir-34^null has adult brain | conditional phenotype, suppressible | partially by Eip74EF^BG01805

(Liu et al., 2012)

NOT suppressed by

Statement

Reference

Fmr1^+tLa, mir-277^+tLa, mir-34^null has adult brain | conditional phenotype, non-suppressible by Eip74EF[+]/Eip74EF^BG01805

(Liu et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous Eip74EF^BG01805 partially suppresses the age-associated defects seen in mir-34^null mutants (expressing mir-277^+tLa and Fmr1^+tLa to restore mir-277 and Fmr1 function respectively). The shortened lifespan and increased brain vacuolisation are both rescued. One copy of Eip74EF^BG01805 is unable to suppress these phenotypes.

(Liu et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

mir-34^null is partially rescued by mir-34^+tLa

(Liu et al., 2012)

Comments

Expression of mir-34^+tLa partially rescues the age-associated phenotypes seen in mir-34^null mutants (expressing mir-277^+tLa and Fmr1^+tLa to restore mir-277 and Fmr1 function respectively).

(Liu et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mir-34 null

(McNeill et al., 2020)

mir-34^null

(Sreejith et al., 2025)

mir-34^Δ

(Fulga et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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