FlyBase Allele Report: Dmel\Atg8a[d4]

General Information

Symbol

Dmel\Atg8a^d4

Species

D. melanogaster

Name

FlyBase ID

FBal0278389

Feature type

allele

Associated gene

Dmel\Atg8a

Associated Insertion(s)

Carried in Construct

Also Known As

Atg8a[d4]

Key Links

Genomic Maps

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Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Pircs et al., 2012)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

deletion

X:10,767,996..10,768,878 [-]

Comment:

Deletion resulting from the imprecise excsion of P{SUPor-P}Atg8a^KG07569, which removes the first 25 aa of Atg8a and the first 53 aa of BTBD9.

(Pircs et al., 2012)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

P{SUPor-P}Atg8a^KG07569

(Pircs et al., 2012)

Caused by aberration

Df(1)Atg8a^d4

Cytology

Nature of the lesion

Statement

Reference

Imprecise excision of P{SUPor-P}Atg8a^KG07569 removes the first 25 codons of Atg8a and the first 53 codons of BTBD9.

(Pircs et al., 2012)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by scrib⁶⁷³, Ras85D^V12.UAS

(Katheder et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Atg8a^d4 mutant adults do not display any obvious eye morphology defects or signs of degeneration.

(Lo Piccolo et al., 2017)

Atg8a^d4 homozygous mutants do not show any obvious defects in the morphology or structure of the adult thorax or loss of thoracic muscles.

(Rui et al., 2015)

Df(1)Atg8a^d4 mutants are homozygous viable and fertile with no gross morphological or developmental abnormalities.

(Pircs et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg8a^d4/Atg8a[+], Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has visible | adult stage phenotype, suppressible by lncRNA:Hsrω^{dsRNA.Sym.UAS}, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

NOT Enhancer of

Statement

Reference

Atg8a^d4/Atg8a[+] is a non-enhancer of visible | adult stage phenotype of Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Suppressor of

Statement

Reference

Atg8a^d4/Atg8a^d4 is a suppressor of abnormal size | somatic clone | third instar larval stage phenotype of Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg8a^d4/Atg8a^d4 is a suppressor of neoplasia | somatic clone | third instar larval stage phenotype of Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg8a^d4 is a suppressor of abnormal size phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

Atg8a^d4 is a suppressor of visible phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

NOT Suppressor of

Statement

Reference

Atg8a^d4/Atg8a^{UAS.GFP,mCherry}, Scer\GAL4^ptc-559.1 is a non-suppressor of abnormal size phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

Atg8a^d4/Atg8a^{UAS.GFP,mCherry}, Scer\GAL4^ptc-559.1 is a non-suppressor of visible phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Atg8a^d4/Atg8a[+], Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has eye phenotype, suppressible by lncRNA:Hsrω^{dsRNA.Sym.UAS}, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

NOT Enhancer of

Statement

Reference

Atg8a^d4/Atg8a[+] is a non-enhancer of eye phenotype of Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Suppressor of

Statement

Reference

Atg8a^d4 is a suppressor of 1st posterior cell phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

Atg8a^d4 is a suppressor of wing basal cell 1 phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

NOT Suppressor of

Statement

Reference

Atg8a^d4/Atg8a^{UAS.GFP,mCherry}, Scer\GAL4^ptc-559.1 is a non-suppressor of 1st posterior cell phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

Atg8a^d4/Atg8a^{UAS.GFP,mCherry}, Scer\GAL4^ptc-559.1 is a non-suppressor of wing basal cell 1 phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

Other

Statement

Reference

Atg8a^d4/Atg8a[+], Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+] has adult thorax phenotype

(Rui et al., 2015)

Atg8a^d4/Atg8a[+], Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+] has somatic muscle cell of thorax phenotype

(Rui et al., 2015)

Atg8a^d4, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307 has adult thorax phenotype

(Rui et al., 2015)

Atg8a^d4, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307 has somatic muscle cell of thorax phenotype

(Rui et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

scrib⁶⁷³-homozygous, Ras85D^V12.Scer\UAS-expressing tumors grow to significantly smaller sizes when grafted into the abdomen of Atg8a^d4 homozygous adult hosts, as compared to control hosts.

(Katheder et al., 2017)

Xenogenetic Interactions

Statement

Reference

The eye degeneration induced by Hsap\FUS^Scer\UAS.cIa expression (one copy) under Scer\GAL4^GMR.PS is not exacerbated by combination with Atg8a^d4/+ and the eye defects in the Scer\GAL4^GMR.PS/Atg8a^d4;Hsap\FUS^Scer\UAS.cIa/+ flies can still be rescued by expression of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} (one copy).

(Lo Piccolo et al., 2017)

Expression of Hsap\MAPT^{Scer\UAS.T:Avic\GFP} in Atg8a^d4 heterozygous background does not produce any obvious phenotype in the adult thorax. However, when Hsap\MAPT^{Scer\UAS.T:Avic\GFP} is expressed in either htt^98E2/+;Atg8a^d4/+ double heterozygotes or Atg8a^d4 homozygotes nearly all the adult flies have collapsed thoraces and display loss of skeletal thoracic muscles.

(Rui et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Atg8a^d4

(Jipa et al., 2021, Billes et al., 2018, Csizmadia et al., 2018, Hussain et al., 2018, Nagy et al., 2014, Pircs et al., 2012)

Atg8a^mt

(Lo Piccolo et al., 2017)

Atg8a[d4]

(Nagy et al., 2015, Nagy et al., 2013)

atg8^d4

(Katheder et al., 2017)

atg8a^d4

(Rui et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (11)

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