FlyBase Allele Report: Dmel\Atg8a[d4]

General Information

Symbol

Dmel\Atg8a^d4

Species

D. melanogaster

Name

FlyBase ID

FBal0278389

Feature type

allele

Associated gene

Dmel\Atg8a

Associated Insertion(s)

Carried in Construct

Also Known As

Atg8a[d4]

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Pircs et al., 2012)

Progenitor genotype

P{SUPor-P}Atg8a^KG07569

(Pircs et al., 2012)

Caused by aberration

Df(1)Atg8a^d4

Cytology

Description

Imprecise excision of P{SUPor-P}Atg8a^KG07569 removes the first 25 codons of Atg8a and the first 53 codons of BTBD9.

(Pircs et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:10,767,996..10,768,878 [-]

Comment:

Deletion resulting from the imprecise excsion of P{SUPor-P}Atg8a^KG07569, which removes the first 25 aa of Atg8a and the first 53 aa of BTBD9.

(Pircs et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by scrib⁶⁷³, Ras85D^V12.UAS

(Katheder et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | P-stage

(Tan et al., 2024)

abnormal wound healing | adult stage

(Szabó et al., 2023)

Phenotype Manifest In

adult marginal nerve wrapping glial cell

(Szabó et al., 2023)

dendrite | P-stage

(Tan et al., 2024)

larval dorsal multidendritic neuron ddaC | P-stage

(Tan et al., 2024)

marginal nerve

(Szabó et al., 2023)

Detailed Description

Statement

Reference

Atg8a^d4 mutant adults do not display any obvious eye morphology defects or signs of degeneration.

(Lo Piccolo et al., 2017)

Atg8a^d4 homozygous mutants do not show any obvious defects in the morphology or structure of the adult thorax or loss of thoracic muscles.

(Rui et al., 2015)

Df(1)Atg8a^d4 mutants are homozygous viable and fertile with no gross morphological or developmental abnormalities.

(Pircs et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg8a^d4/Atg8a[+], Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has visible | adult stage phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

NOT Enhancer of

Statement

Reference

Atg8a^d4 is a non-enhancer of abnormal neuroanatomy | P-stage phenotype of Scer\GAL4^ppk.PU, cnc^GD4437

(Tan et al., 2024)

Atg8a^d4/Atg8a[+] is a non-enhancer of visible | adult stage phenotype of Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Suppressor of

Statement

Reference

Atg8a^d4/Atg8a^d4 is a suppressor of neoplasia | somatic clone | third instar larval stage phenotype of Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg8a^d4/Atg8a^d4 is a suppressor of abnormal size | somatic clone | third instar larval stage phenotype of Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg8a^d4 is a suppressor of abnormal size phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Atg8a^d4 is a suppressor of visible phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

NOT Suppressor of

Statement

Reference

Atg8a^d4 is a non-suppressor of abnormal neuroanatomy | P-stage phenotype of Scer\GAL4^ppk.PU, cnc^GD4437

(Tan et al., 2024)

Atg8a^d4/Atg8a^{UAS.GFP,mCherry}, Scer\GAL4^ptc-559.1 is a non-suppressor of abnormal size phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Atg8a^d4/Atg8a^{UAS.GFP,mCherry}, Scer\GAL4^ptc-559.1 is a non-suppressor of visible phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Atg8a^d4/Atg8a[+], Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has eye phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

NOT Enhancer of

Statement

Reference

Atg8a^d4 is a non-enhancer of larval dorsal multidendritic neuron ddaC | P-stage phenotype of Scer\GAL4^ppk.PU, cnc^GD4437

(Tan et al., 2024)

Atg8a^d4 is a non-enhancer of dendrite | P-stage phenotype of Scer\GAL4^ppk.PU, cnc^GD4437

(Tan et al., 2024)

Atg8a^d4/Atg8a[+] is a non-enhancer of eye phenotype of Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Suppressor of

Statement

Reference

Atg8a^d4 is a suppressor of first posterior wing cell phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Atg8a^d4 is a suppressor of first basal wing cell phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

NOT Suppressor of

Statement

Reference

Atg8a^d4 is a non-suppressor of larval dorsal multidendritic neuron ddaC | P-stage phenotype of Scer\GAL4^ppk.PU, cnc^GD4437

(Tan et al., 2024)

Atg8a^d4 is a non-suppressor of dendrite | P-stage phenotype of Scer\GAL4^ppk.PU, cnc^GD4437

(Tan et al., 2024)

Atg8a^d4/Atg8a^{UAS.GFP,mCherry}, Scer\GAL4^ptc-559.1 is a non-suppressor of first posterior wing cell phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Atg8a^d4/Atg8a^{UAS.GFP,mCherry}, Scer\GAL4^ptc-559.1 is a non-suppressor of first basal wing cell phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Nagy et al., 2013)

Other

Statement

Reference

Atg8a^d4/Atg8a[+], Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+] has adult thorax phenotype

(Rui et al., 2015)

Atg8a^d4/Atg8a[+], Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+] has adult somatic muscle cell of thorax phenotype

(Rui et al., 2015)

Atg8a^d4, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307 has adult thorax phenotype

(Rui et al., 2015)

Atg8a^d4, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307 has adult somatic muscle cell of thorax phenotype

(Rui et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

scrib⁶⁷³-homozygous, Ras85D^V12.Scer\UAS-expressing tumors grow to significantly smaller sizes when grafted into the abdomen of Atg8a^d4 homozygous adult hosts, as compared to control hosts.

(Katheder et al., 2017)

Xenogenetic Interactions

Statement

Reference

The eye degeneration induced by Hsap\FUS^Scer\UAS.cIa expression (one copy) under Scer\GAL4^GMR.PS is not exacerbated by combination with Atg8a^d4/+ and the eye defects in the Scer\GAL4^GMR.PS/Atg8a^d4;Hsap\FUS^Scer\UAS.cIa/+ flies can still be rescued by expression of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} (one copy).

(Lo Piccolo et al., 2017)

Expression of Hsap\MAPT^{Scer\UAS.T:Avic\GFP} in Atg8a^d4 heterozygous background does not produce any obvious phenotype in the adult thorax. However, when Hsap\MAPT^{Scer\UAS.T:Avic\GFP} is expressed in either htt^98E2/+;Atg8a^d4/+ double heterozygotes or Atg8a^d4 homozygotes nearly all the adult flies have collapsed thoraces and display loss of skeletal thoracic muscles.

(Rui et al., 2015)

Complementation and Rescue Data

Rescued by

Atg8a^d4 is rescued by Atg8a^3x.mCherry

(Szabó et al., 2023)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Atg8a^d4

(Tan et al., 2024, Jipa et al., 2021, Billes et al., 2018, Csizmadia et al., 2018, Hussain et al., 2018, Nagy et al., 2014, Pircs et al., 2012)

Atg8a^mt

(Lo Piccolo et al., 2017)

Atg8a^Δ4

(Szabó et al., 2023)

Atg8a[d4]

(Nagy et al., 2015, Nagy et al., 2013)

atg8^d4

(Katheder et al., 2017)

atg8a^d4

(Jain et al., 2015, Rui et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (14)

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