FlyBase Allele Report: Dmel\Atg8a[d4]

General Information

Symbol

Dmel\Atg8a^d4

Species

D. melanogaster

Name

FlyBase ID

FBal0278389

Feature type

allele

Associated gene

Dmel\Atg8a

Associated Insertion(s)

Carried in Construct

Also Known As

Atg8a[d4]

Key Links

Genomic Maps

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Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Pircs et al., 2012)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

deletion

X:10,767,996..10,768,878 [-]

Comment:

Deletion resulting from the imprecise excsion of P{SUPor-P}Atg8a^KG07569, which removes the first 25 aa of Atg8a and the first 53 aa of BTBD9.

(Pircs et al., 2012)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

P{SUPor-P}Atg8a^KG07569

(Pircs et al., 2012)

Cytology

Nature of the lesion

Statement

Reference

Imprecise excision of P{SUPor-P}Atg8a^KG07569 removes the first 25 codons of Atg8a and the first 53 codons of BTBD9.

(Pircs et al., 2012)

Caused by aberration

Df(1)Atg8a^d4

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by scrib⁶⁷³, Ras85D^V12.UAS

(Katheder et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Atg8a^d4 mutant adults do not display any obvious eye morphology defects or signs of degeneration.

(Lo Piccolo et al., 2017)

Atg8a^d4 homozygous mutants do not show any obvious defects in the morphology or structure of the adult thorax or loss of thoracic muscles.

(Rui et al., 2015)

Df(1)Atg8a^d4 mutants are homozygous viable and fertile with no gross morphological or developmental abnormalities.

(Pircs et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg8a^d4/Atg8a[+], Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has visible | adult stage phenotype, suppressible by lncRNA:Hsrω^{dsRNA.Sym.UAS}, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

NOT Enhancer of

Statement

Reference

Atg8a^d4/Atg8a[+] is a non-enhancer of visible | adult stage phenotype of Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Suppressor of

Statement

Reference

Atg8a^d4/Atg8a^d4 is a suppressor of abnormal size | somatic clone | third instar larval stage phenotype of Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg8a^d4/Atg8a^d4 is a suppressor of neoplasia | somatic clone | third instar larval stage phenotype of Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg8a^d4 is a suppressor of abnormal size phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

Atg8a^d4 is a suppressor of visible phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

NOT Suppressor of

Statement

Reference

Atg8a^d4/Atg8a^{UAS.GFP,mCherry}, Scer\GAL4^ptc-559.1 is a non-suppressor of abnormal size phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

Atg8a^d4/Atg8a^{UAS.GFP,mCherry}, Scer\GAL4^ptc-559.1 is a non-suppressor of visible phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Atg8a^d4/Atg8a[+], Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has eye phenotype, suppressible by lncRNA:Hsrω^{dsRNA.Sym.UAS}, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

NOT Enhancer of

Statement

Reference

Atg8a^d4/Atg8a[+] is a non-enhancer of eye phenotype of Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Suppressor of

Statement

Reference

Atg8a^d4 is a suppressor of 1st posterior cell phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

Atg8a^d4 is a suppressor of wing basal cell 1 phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

NOT Suppressor of

Statement

Reference

Atg8a^d4/Atg8a^{UAS.GFP,mCherry}, Scer\GAL4^ptc-559.1 is a non-suppressor of 1st posterior cell phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

Atg8a^d4/Atg8a^{UAS.GFP,mCherry}, Scer\GAL4^ptc-559.1 is a non-suppressor of wing basal cell 1 phenotype of Scer\GAL4^ptc-559.1, Myc^UAS.cZa

(Nagy et al., 2013)

Other

Statement

Reference

Atg8a^d4/Atg8a[+], Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+] has adult thorax phenotype

(Rui et al., 2015)

Atg8a^d4/Atg8a[+], Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+] has skeletal muscle of thorax phenotype

(Rui et al., 2015)

Atg8a^d4, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307 has adult thorax phenotype

(Rui et al., 2015)

Atg8a^d4, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307 has skeletal muscle of thorax phenotype

(Rui et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

scrib⁶⁷³-homozygous, Ras85D^V12.Scer\UAS-expressing tumors grow to significantly smaller sizes when grafted into the abdomen of Atg8a^d4 homozygous adult hosts, as compared to control hosts.

(Katheder et al., 2017)

Xenogenetic Interactions

Statement

Reference

The eye degeneration induced by Hsap\FUS^Scer\UAS.cIa expression (one copy) under Scer\GAL4^GMR.PS is not exacerbated by combination with Atg8a^d4/+ and the eye defects in the Scer\GAL4^GMR.PS/Atg8a^d4;Hsap\FUS^Scer\UAS.cIa/+ flies can still be rescued by expression of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} (one copy).

(Lo Piccolo et al., 2017)

Expression of Hsap\MAPT^{Scer\UAS.T:Avic\GFP} in Atg8a^d4 heterozygous background does not produce any obvious phenotype in the adult thorax. However, when Hsap\MAPT^{Scer\UAS.T:Avic\GFP} is expressed in either htt^98E2/+;Atg8a^d4/+ double heterozygotes or Atg8a^d4 homozygotes nearly all the adult flies have collapsed thoraces and display loss of skeletal thoracic muscles.

(Rui et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Atg8a^d4

(Billes et al., 2018, Csizmadia et al., 2018, Hussain et al., 2018, Nagy et al., 2014, Pircs et al., 2012)

Atg8a^mt

(Lo Piccolo et al., 2017)

Atg8a[d4]

(Nagy et al., 2015, Nagy et al., 2013)

atg8^d4

(Katheder et al., 2017)

atg8a^d4

(Rui et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (10)

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