Genomic fragment containing polo tagged at the 5' end with GFP. The second, more distal, polyadenylation site (pA2) in the 3'UTR (and the polo sequence downstream of it) has been deleted, resulting in the expression of only the shorter polo transcript which utilizes the first, more proximal, polyadenylation site (pA1) in the 3'UTR.
poloΔpA2.GFP fails to rescue poloS025604
Nearly all polopA2.T:Avic\GFP;poloS025604 flies die during pupariation.