FlyBase Allele Report: Dmel\polo[GFP]

General Information

Symbol

Dmel\polo^GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0117398

Feature type

allele

Associated gene

Dmel\polo

Associated Insertion(s)

Carried in Construct

P{GFP-polo}

Also Known As

GFP-polo, Polo-GFP

Key Links

Transgenic product class

genomic_DNA

(Moutinho-Santos et al., 1999)

wild_type

(Moutinho-Santos et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Moutinho-Santos et al., 1999)

wild_type

(Moutinho-Santos et al., 1999)

Mutagen

in vitro construct

(Moutinho-Santos et al., 1999)

Progenitor genotype

Carried in construct

P{GFP-polo}

Cytology

Description

Genomic fragment containing polo tagged at the 5' end with GFP.

(Moutinho-Santos et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

polo

Encoded product / tool

polo

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | oogenesis

(Mirouse et al., 2006)

lethal - all die before end of pupal stage, with polo^S025604

(Pinto et al., 2011)

some die during pupal stage, with polo^S025604

(Pinto et al., 2011)

wild-type

(Moutinho-Santos et al., 1999)

Phenotype Manifest In

abdominal tergite, with polo^S025604

(Pinto et al., 2011)

adult abdomen, with polo^S025604

(Pinto et al., 2011)

female germline cyst

(Mirouse et al., 2006)

germarium

(Mirouse et al., 2006)

Detailed Description

Statement

Reference

Analysis of the few polo^{pA2.T:Avic\GFP};polo^S025604 escapers indicates that these individuals exhibit strong defects in abdominal morphology, with the complete absence or incorrect formation of tergites, while all other adult structures appear normal.

Only large polytene larval cells are observed in the epidermis of polo^{pA2.T:Avic\GFP};polo^S025604/polo^S025604 pupa. No histoblasts are detected in polo^{pA2.T:Avic\GFP};polo^S025604/polo^S025604 pupa, but are present in larvae.

(Pinto et al., 2011)

Females carrying polo^T:Avic\GFP can show initiation of meiosis in more than four cells in the germarium and cysts in region 3 still contain four cells in meiosis, indicating a strong delay in the restriction of meiosis to one cell.

(Mirouse et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

polo^GFP is a suppressor of abnormal neuroanatomy phenotype of mts²⁹⁹

(Wang et al., 2009)

Phenotype Manifest In