FlyBase Allele Report: Dmel\boi[KO]

General Information

Symbol

Dmel\boi^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0280268

Feature type

allele

Associated gene

Dmel\boi

Associated Insertion(s)

TI{TI}boi^KO

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Zheng et al., 2010)

Mutagen

FLPase

(Zheng et al., 2010)

I-SceI

(Zheng et al., 2010)

ends-out gene targeting

(Zheng et al., 2010)

Progenitor genotype

Associated Insertion(s)

TI{TI}boi^KO

Cytology

Description

A 1586-base-pair contiguous genomic segment containing the entire fourth exon and a portion of the fifth exon of boi have been replaced by a w^+mW.hs marker. The mutation results in the deletion of the AUG initiation codon of boi, the signal sequence, and the four Ig domains from the coding sequence. The z gene (which is nested within boi) is intact.

(Zheng et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Zheng et al., 2010)

viable

(Zheng et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

boi^KO is found to be homozygous viable and fertile.

(Zheng et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

boi^KO is a suppressor of viable phenotype of ihog^GLC

(Zheng et al., 2010)

Phenotype Manifest In

Other

Statement

Reference

boi^KO, ihog^GLC has ventral denticle belt phenotype

(Zheng et al., 2010)

boi^KO, ihog^GLC has embryonic/first instar larval cuticle phenotype

(Zheng et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Animals bred to homozygosity for both ihog^GLC and boi^KO die in early larval stages.

Embryos derived from maternal germline clones of ihog^GLC in a boi^KO homozygous mutant background show a cuticle phenotype. Cuticle preparations from the mutant embryos reveal a fully penetrant phenotype in which naked cuticle normally present in the posterior of each segment fails to develop, resulting in a continuous lawn of poorly patterned denticles.

(Zheng et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

boi^KO

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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