FlyBase Allele Report: Hsap\MAPT[UAS.S11A]

General Information

Symbol

Hsap\MAPT^UAS.S11A

Species

H. sapiens

Name

FlyBase ID

FBal0281835

Feature type

allele

Associated gene

Hsap\MAPT

Associated Insertion(s)

Carried in Construct

P{UAS-Tau.S11A}

Key Links

Transgenic product class

missense_variant

(Chatterjee et al., 2009)

polypeptide_post_translational_processing_variant

(Chatterjee et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Chatterjee et al., 2009)

polypeptide_post_translational_processing_variant

(Chatterjee et al., 2009)

Mutagen

in vitro construct

(Chatterjee et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Tau.S11A}

Cytology

Description

UAS regulates expression of an oligonucleotide encoding wild type Hsap\MAPT with mutations at 11 of 14 major serine/threonine sgg phosphorylation sites: S46A, T50A, S199A, T202A, T205A, T212A, S214A, T231A, S235A, S396A and S404A. The two amino terminal inserts generated by alternative splicing of Hsap\MAPT exons 2 and 3 are included.

(Chatterjee et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\MAPT

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Bougé and Parmentier, 2016)

Phenotype Manifest In

mitotic spindle | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Bougé and Parmentier, 2016)

wing disc | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Bougé and Parmentier, 2016)

Detailed Description

Statement

Reference

Expression of Hsap\MAPT^{Scer\UAS.S11A} under the control of Scer\GAL4^Bx-MS1096 induces aberrant monopolar mitotic spindles in the third instar larval wing disc cells.

(Bougé and Parmentier, 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\MAPT^UAS.S11A, Scer\GAL4^GMR.PS has visible phenotype, suppressible by Dlic^G0190/Dlic[+]

(Ambegaokar and Jackson, 2011)

Hsap\MAPT^UAS.S11A, Scer\GAL4^GMR.PS has visible phenotype, suppressible by ksr^j5E2/ksr[+]

(Ambegaokar and Jackson, 2011)

Hsap\MAPT^UAS.S11A, Scer\GAL4^GMR.PS has visible phenotype, suppressible by NC2α^EY18723/NC2alpha[+]

(Ambegaokar and Jackson, 2011)

Hsap\MAPT^UAS.S11A, Scer\GAL4^GMR.PS has visible phenotype, suppressible by SdhB^EY12081, Scer\GAL4^GMR.PS

(Ambegaokar and Jackson, 2011)

Hsap\MAPT^UAS.S11A, Scer\GAL4^GMR.PS has visible phenotype, suppressible by sgg^G0335/sgg[+]

(Ambegaokar and Jackson, 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\MAPT^UAS.S11A, Scer\GAL4^GMR.PS has eye phenotype, suppressible by Dlic^G0190/Dlic[+]

(Ambegaokar and Jackson, 2011)

Hsap\MAPT^UAS.S11A, Scer\GAL4^GMR.PS has eye phenotype, suppressible by ksr^j5E2/ksr[+]

(Ambegaokar and Jackson, 2011)

Hsap\MAPT^UAS.S11A, Scer\GAL4^GMR.PS has eye phenotype, suppressible by NC2α^EY18723/NC2alpha[+]

(Ambegaokar and Jackson, 2011)

Hsap\MAPT^UAS.S11A, Scer\GAL4^GMR.PS has eye phenotype, suppressible by SdhB^EY12081, Scer\GAL4^GMR.PS

(Ambegaokar and Jackson, 2011)

Hsap\MAPT^UAS.S11A, Scer\GAL4^GMR.PS has eye phenotype, suppressible by sgg^G0335/sgg[+]

(Ambegaokar and Jackson, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

One copy of Dlic^G0190 suppresses the rough eye phenotype seen in flies expressing Hsap\MAPT^{Scer\UAS.S11A} under the control of Scer\GAL4^GMR.PS.

One copy of ksr^j5E2 suppresses the rough eye phenotype seen in flies expressing Hsap\MAPT^{Scer\UAS.S11A} under the control of Scer\GAL4^GMR.PS.

One copy of NC2α^EY18723 suppresses the rough eye phenotype seen in flies expressing Hsap\MAPT^{Scer\UAS.S11A} under the control of Scer\GAL4^GMR.PS.

Expression of SdhB^EY12081 suppresses the rough eye phenotype seen in flies expressing Hsap\MAPT^{Scer\UAS.S11A} under the control of Scer\GAL4^GMR.PS.

One copy of sgg^G0335 suppresses the rough eye phenotype seen in flies expressing Hsap\MAPT^{Scer\UAS.S11A} under the control of Scer\GAL4^GMR.PS.

(Ambegaokar and Jackson, 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51366

w¹¹¹⁸; P{UAS-Tau.S11A}1.2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\MAPT^{Scer\UAS.S11A}

Hsap\MAPT^UAS.S11A

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close