FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4ple.TH-D-4
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General Information
Symbol
Scer\GAL4ple.TH-D-4
Species
S. cerevisiae
Name
FlyBase ID
FBal0282527
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
P{ple-GAL4.TH-D}4
Carried in Construct
Also Known As
TH-D4-Gal4, TH-D4
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Liu et al., 2012)
Progenitor genotype
Scer\GAL4ple.TH-D
(Liu et al., 2012)
Associated Insertion(s)
P{ple-GAL4.TH-D}4
Cytology
Description

Insertion of P{ple-GAL4.TH-D} resulting in a line-specific expression pattern.

(Liu et al., 2012)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      dopaminergic PPM2 neuron | subset
      (Liu et al., 2012)
      dopaminergic PPM3 neuron | subset
      (Liu et al., 2012)
      mFB neuron of the dopaminergic PPM3 cluster
      (Liu et al., 2012)
      vFB neuron of the dopaminergic PPM3 cluster | faint
      (Liu et al., 2012)
      EB neuron of the dopaminergic PPM3 cluster
      (Liu et al., 2012)
      dopaminergic PPL1 neuron | subset
      (Liu et al., 2012)
      DP neuron of the dopaminergic PPL1 cluster
      (Liu et al., 2012)
      MB-alpha neuron of the dopaminergic PPL1 cluster | faint
      (Liu et al., 2012)
      dFB neuron of the dopaminergic PPL1 cluster
      (Liu et al., 2012)
      mushroom body pedunculus-medial lobe arborizing neuron 1
      (Liu et al., 2012)
      mushroom body vertical lobe arborizing neuron 1
      (Liu et al., 2012)
      Additional Information
      Statement
      Reference

      ScerGAL4ple.TH-D' drives expression in a subset of the dopaminergic neurons in the adult brain labelled by ScerGAL4ple.PF : around 1 PPM2 neurons, 3 PPM3 neurons (of the mFB, vFB and EB types) and 5 PPL1 neurons (of the DP, MB-V1, MP-MP1, MB-alpha and dFB types).

      (Liu et al., 2012)
       
      Marker for
      Reflects expression of
      ple
      (Liu et al., 2012)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Activation of neurons expressing any of the specific dopaminergic neuron drivers Scer\GAL4ple.TH-D, Scer\GAL4ple.TH-D-4 or Scer\GAL4ple.TH-F-1 through overexpression of temperature sensitive TrpA1 results in increased sleep loss in both mature (7-8 day) flies compared to controls. Expression under the control of Scer\GAL4ple.TH-D or Scer\GAL4ple.TH-D-4 also results in sleep loss in young (1 day old) flies. The resulting Scer\GAL4ple.TH-D-4>TrpA1 mature flies show a deficit in courtship measures and a reduction in VA1v volume. DA1 and VA1d volumes are unaffected. No difference in sleep amount in young flies is seen when neurons expressing Scer\GAL4ple.TH-F-1 are activated. Activating neurons that express in ventral projections to the antennal lobe (AL) in young flies using Scer\GAL4ple.TH-C results in sleep loss, but the flies do not show courtship deficits or glomerular volumetric changes in mature adulthood.

      (Kayser et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4ple.TH-D-4
      Name Synonyms
      Secondary FlyBase IDs
        References (7)