In the adult brain, ScerGAL4394 drives intense expression in the optic lobes, in circular structures near the mushroom body and in snow-drop shaped structures (SD) in the lateral protocerebrum. In the larval brain, expression is detected in two neurons in the anterior medial region of each lobe.
Scer\GAL4394 homozygous males produce songs with abnormally long interpulse intervals.