FB2021_01, released Feb 18, 2021

Allele: Scer\GAL4CrzR.3.5

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General Information
Symbol
Scer\GAL4CrzR.3.5
Species
S. cerevisiae
Name
FlyBase ID
FBal0283230
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{CrzR-GAL4.3.5}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Tayler et al., 2012)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{CrzR-GAL4.3.5}
      Cytology
      Nature of the lesion
      Statement
      Reference

      A 3.5kb fragment from upstream of the CrzR putative transcriptional start site is fused upstream of sequence encoding a GAL4 driver.

      (Tayler et al., 2012)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      CrzR
      Encoded product / tool
      GAL4
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      interneuron | subset of adult abdominal neuromere
      (Tayler et al., 2012)
      ejaculatory duct
      (Tayler et al., 2012)
      male accessory gland
      (Tayler et al., 2012)
      seminal vesicle
      (Tayler et al., 2012)
      Additional Information
      Statement
      Reference

      Scer\GAL4CrzR.3.5 drives expression in 2 pairs of interneurons in the abdominal ganglion, and in the male reproductive system organs. In the abdominal ganglion, expression of Scer\GAL4CrzR.3.5 is closely intermingled with the expression of Crz.

      (Tayler et al., 2012)
       
      Marker for
      Reflects expression of
      CrzR
      (Tayler et al., 2012)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 650 nm (red) light) of Scer\GAL4CrzR.3.5 cells induces ejaculation in males and results in a significant preference for an illuminated zone over non-illuminated areas, which is not seen in controls.

      (Zer-Krispil et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4CrzR.3.5
      Name Synonyms
      Secondary FlyBase IDs
        References (3)