A 3.5kb fragment from upstream of the CrzR putative transcriptional start site is fused upstream of sequence encoding a GAL4 driver.
Scer\GAL4CrzR.3.5 drives expression in 2 pairs of interneurons in the abdominal ganglion, and in the male reproductive system organs. In the abdominal ganglion, expression of Scer\GAL4CrzR.3.5 is closely intermingled with the expression of Crz.
abnormal mating | male limited | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus
abnormal phototaxis | adult stage | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus
male sterile, with Hsap\KCNJ2UAS.EGFP
Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 650 nm (red) light) of Scer\GAL4CrzR.3.5 cells induces ejaculation in males and results in a significant preference for an illuminated zone over non-illuminated areas, which is not seen in controls.