FlyBase Allele Report: Dmel\Tsp66E[2]

General Information

Symbol

Dmel\Tsp66E²

Species

D. melanogaster

Name

FlyBase ID

FBal0283689

Feature type

allele

Associated gene

Dmel\Tsp66E

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Han et al., 2012)

Mutagen

P-element activity

(Han et al., 2012)

Progenitor genotype

P{EPgy2}Tsp66E^EY02264

(Han et al., 2012)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes part of Tsp66E.

(Han et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | maternal effect

(Han et al., 2012)

some die during embryonic stage | maternal effect

(Han et al., 2012)

Phenotype Manifest In

egg | maternal effect

(Han et al., 2012)

Detailed Description

Statement

Reference

The hatching rate of eggs laid by homozygous females is significantly reduced compared to wild type, although the number of eggs laid is similar to wild type. The eggs are reduced in length.

(Han et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Tsp74F[+]/Tsp74F¹, Tsp66E²/Tsp66E² is an enhancer of visible phenotype of if³

(Han et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Tsp66E² has egg | maternal effect phenotype, enhanceable by if³

(Han et al., 2012)

Suppressed by

Statement

Reference

Tsp66E² has egg | maternal effect phenotype, suppressible by Hsap\CD82^UAS.Tag:HA/Scer\GAL4^da.G32

(Han et al., 2012)

Enhancer of

Statement

Reference

Tsp74F[+]/Tsp74F¹, Tsp66E²/Tsp66E² is an enhancer of wing phenotype of if³

(Han et al., 2012)

Other

Statement

Reference

Tsp66E², Tsp74F¹ has actin filament phenotype

(Han et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

At stage 12 of oogenesis, alignment of actin filament bundles in the follicle cells of Tsp66E² Tsp74F¹ double mutant females is severely disturbed. Endocytosis is severely reduced (assayed by dextran uptake) in the mutant follicle cells at this stage.

if³ enhances the short-egg phenotype seen in eggs laid by Tsp66E² females.

The penetrance of the if³ wing blister phenotype is enhanced if the flies also carry Tsp66E²/Tsp66E² Tsp74F¹.

(Han et al., 2012)

Xenogenetic Interactions

Statement

Reference

The expression of Hsap\CD82^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 significantly rescues the elongation defects seen in eggs laid by Tsp66E² females.

(Han et al., 2012)

Complementation and Rescue Data

Rescued by

Tsp66E² is rescued by Scer\GAL4^da.G32/Tsp66E^UAS.Tag:HA

(Han et al., 2012)

Comments

The expression of Tsp66E^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 significantly rescues the elongation defects seen in eggs laid by Tsp66E² females.

(Han et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Tsp66E²

(Han et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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