FlyBase Allele Report: Dmel\sicily[UAS.cZa]

General Information

Symbol

Dmel\sicily^UAS.cZa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Zhang

FlyBase ID

FBal0284117

Feature type

allele

Associated gene

Dmel\sicily

Associated Insertion(s)

Carried in Construct

PBac{UAS-sicily.Z}

Also Known As

UAS-sicily

Key Links

Transgenic product class

cDNA

(Zhang et al., 2013)

wild_type

(Zhang et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zhang et al., 2013)

wild_type

(Zhang et al., 2013)

Mutagen

in vitro construct

(Zhang et al., 2013)

Progenitor genotype

Carried in construct

PBac{UAS-sicily.Z}

Cytology

Description

UAS regulatory sequences drive expression of the sicily coding sequence (derived from the RE44923 cDNA clone).

(Zhang et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

sicily

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT ameliorate Parkinson's disease

modeled by park²⁵

(Pogson et al., 2014)

ameliorates Parkinson's disease

modeled by Pink1^B9

(Pogson et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

sicily^UAS.cZa, Scer\GAL4^GMR.PU is a non-enhancer of visible | adult stage | progressive phenotype of Chchd2^S81L.UAS, Scer\GAL4^GMR.PU

(Baek et al., 2021)

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/sicily^UAS.cZa is a suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Pogson et al., 2014)

Scer\GAL4^da.PU/sicily^UAS.cZa is a suppressor of abnormal gravitaxis phenotype of Pink1^B9

(Pogson et al., 2014)

Scer\GAL4^da.PU/sicily^UAS.cZa is a suppressor of abnormal flight phenotype of Pink1^B9

(Pogson et al., 2014)

NOT Suppressor of

Statement

Reference

sicily^UAS.cZa, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage | progressive phenotype of Chchd2^S81L.UAS, Scer\GAL4^GMR.PU

(Baek et al., 2021)

Scer\GAL4^da.PU/sicily^UAS.cZa is a non-suppressor of abnormal flight phenotype of park²⁵

(Pogson et al., 2014)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

sicily^UAS.cZa, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Chchd2^S81L.UAS, Scer\GAL4^GMR.PU

(Baek et al., 2021)

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/sicily^UAS.cZa is a suppressor | partially of flight muscle cell phenotype of Pink1^B9

(Pogson et al., 2014)

Scer\GAL4^da.PU/sicily^UAS.cZa is a suppressor | partially of mitochondrion phenotype of Pink1^B9

(Pogson et al., 2014)

NOT Suppressor of

Statement

Reference

sicily^UAS.cZa, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Chchd2^S81L.UAS, Scer\GAL4^GMR.PU

(Baek et al., 2021)

Scer\GAL4^da.PU/sicily^UAS.cZa is a non-suppressor of mitochondrion | third instar larval stage phenotype of Pink1⁵

(Tsai et al., 2018)

Scer\GAL4^da.PU/sicily^UAS.cZa is a non-suppressor of mitochondrial crista | third instar larval stage phenotype of Pink1⁵

(Tsai et al., 2018)

Scer\GAL4^da.PU/sicily^UAS.cZa is a non-suppressor of embryonic/larval hypodermal muscle cell | third instar larval stage phenotype of Pink1⁵

(Tsai et al., 2018)

Scer\GAL4^da.PU/sicily^UAS.cZa is a non-suppressor of flight muscle cell phenotype of park²⁵

(Pogson et al., 2014)

Scer\GAL4^da.PU/sicily^UAS.cZa is a non-suppressor of mitochondrion phenotype of park²⁵

(Pogson et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Ubiquitous expression of sicily^Scer\UAS.cZa under the control of Scer\GAL4^da.PU restores climbing and flight ability in Pink1^B9 mutant flies. Flight muscle and mitochondrial integrity are only partially restored.

Expression of sicily^Scer\UAS.cZa under the control of Scer\GAL4^da.PU rescues the reduction in complex I seen in Pink1^B9 mutant flies, but ATP levels are not significantly increased.

Ubiquitous expression of sicily^Scer\UAS.cZa under the control of Scer\GAL4^da.PU does not rescue the climbing and flight defects seen in park²⁵ mutant flies. The flight muscle and mitochondrial integrity defects are also not rescued.

Expression of sicily^Scer\UAS.cZa under the control of Scer\GAL4^da.PU does not rescue the reduction in complex I and ATP levels seen in park²⁵ mutant flies.

(Pogson et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^da.PU/sicily^UAS.cZa rescues sicily^A

(Zhang et al., 2013)

Scer\GAL4^da.PU/sicily^UAS.cZa rescues sicily^B

(Zhang et al., 2013)

Scer\GAL4^da.PU/sicily^UAS.cZa rescues sicily^C

(Zhang et al., 2013)