FB2026_02 , released June 18, 2026
Allele: Dmel\poloH518A.K520M.GFP
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General Information
Symbol
Dmel\poloH518A.K520M.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0284273
Feature type
allele
Associated gene
Dmel\polo
Associated Insertion(s)
Carried in Construct
P{GFP-polo.H518A.K520M}
Key Links
Transgenic product class
missense_variant
(Bonner et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Bonner et al., 2013)
Mutagen
in vitro construct
(Bonner et al., 2013)
Progenitor genotype
Carried in construct
P{GFP-polo.H518A.K520M}
Cytology
Description

The polo promoter drives expression of wild-type polo containing the amino acid replacements H518A and K520M. The protein is tagged with GFP.

(Bonner et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
polo
Encoded product / tool
polo
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      poloH518A.K520M.GFP fails to rescue polo1/Df(3L)rdgC-co2

      (Bonner et al., 2013)

      poloH518A.K520M.GFP fails to rescue polo16-1/Df(3L)rdgC-co2

      (Bonner et al., 2013)
      Comments

      poloH518A.K520M.T:Avic\GFP fails to rescue the sterility of polo1/Df(3L)rdgC-co2 females.

      poloH518A.K520M.T:Avic\GFP fails to rescue the lethality of polo16-1/Df(3L)rdgC-co2 animals.

      (Bonner et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      poloH518A.K520M.GFP
      poloH518A.K520M.T:Avic\GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)