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General Information
Symbol
Dmel\Syx17LL06330
Species
D. melanogaster
Name
FlyBase ID
FBal0284931
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Syx17[LL]
Key Links
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Insertion 22 nucleotides downstream of the translation start site.

    Insertion components
    PBac{SAstopDsRed}Syx17LL06330
    Product class / Tool use(s)
    Encoded product / tool
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Syx17LL06330/Df(3L)Exel8098 eye discs have normal morphology. The cells show accumulation of autophagosomes intracellularly, but secreted autophagosomes are not seen.

    Autolysosome formation is inhibited in the fat body cells of starved Syx17LL06330/Df(3L)Exel8098 mutant wandering third instar larvae. Climbing defects are seen in adult flies.

    The eye colour of Syx17LL06330/Df(3L)Exel8098 mutant flies is similar to wild type controls.

    Fat body cells of starved Syx17LL06330/Df(3L)Exel8098 animals accumulate double-membrane autophagosomes and show impaired generation of autolysosomes.

    Syx17LL06330/Syx17f03584 and Syx17LL06330/Df(3L)Exel8098 flies show poor climbing ability compared to controls.

    Brain neurons of well-fed Syx17LL06330/Df(3L)Exel8098 flies show large-scale accumulation of autophagosomes and the brains show apoptotic DNA fragmentation.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Expression of thScer\UAS.cHa under the control of Scer\GAL4elav.PU does not rescue the climbing defects of Syx17LL06330/Df(3L)Exel8098 flies.

    Xenogenetic Interactions
    Statement
    Reference

    Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4elav.PU does not rescue the climbing defects of Syx17LL06330/Df(3L)Exel8098 flies.

    Complementation and Rescue Data
    Comments

    Expression of Syx17+tgen rescues the autolysosome formation defects seen in starved Syx17LL06330/Df(3L)Exel8098 mutant wandering third instar larvae. The climbing defects seen in the resulting adult flies are also rescued.

    Expression of Syx171-301.T:Zzzz\FLAG rescues the autolysosome formation defects seen in starved Syx17LL06330/Df(3L)Exel8098 mutant wandering third instar larvae. The climbing defects seen in the resulting adult flies are also rescued.

    Expression of Syx171-270.T:Zzzz\FLAG rescues the autolysosome formation defects seen in starved Syx17LL06330/Df(3L)Exel8098 mutant wandering third instar larvae. The climbing defects seen in the resulting adult flies are also rescued.

    Expression of Syx171-251.T:Disc\RFP-mCherry fails to rescue the autolysosome formation defects seen in starved Syx17LL06330/Df(3L)Exel8098 mutant wandering third instar larvae. The climbing defects seen in the resulting adult flies are also not rescued.

    Syx17+tgen rescues the climbing defects of Syx17LL06330/Df(3L)Exel8098 flies.

    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (6)
    References (9)