UASp regulatory sequences are fused upstream of sequences encoding the Ada2b S isoform, including the Ada2b regulatory region. The Ada2b coding sequence is tagged at the C-terminal end with EGFP. The protein can be expressed either under the control of its own regulatory sequences or, if a GAL4 driver is used, under the control of the UASp regulatory sequences.
Ada2bUASp.S.EGFP partially rescues Ada2bd842
10% of Ada2bd842 mutants carrying a single copy of Ada2bScer\UAS.P\T.S.T:Avic\GFP-EGFP emerge as adults.