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General Information
Symbol
Dmel\TBPHUAS.cDa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Diaper
FlyBase ID
FBal0287629
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-TBPH
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a TBPH cDNA (GH09868).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Motor neuron clones expressing TBPHScer\UAS.cDa under the control of Scer\GAL4VGlut-OK371 exhibit clear motor axon and NMJ degeneration.

Expression of TBPHScer\UAS.cDa under the control of Scer\GAL4Mef2.PR results in premature lethality, with the majority of animals dying during the second larval instar and none developing later than the third larval instar. Mutant second instar larvae show significantly impaired body wall peristalsis compared to controls. Sarcoplasmic aggregates containing TBPH protein are seen in the body wall muscles of mutant second instar larvae.

Animals expressing TBPHScer\UAS.cDa under the control of Scer\GAL4repo die during larval stages.

Only around 10% of flies expressing TBPHScer\UAS.cDa under the control of Scer\GAL4elav.PU eclose as adults. The majority of flies die during late larval/pupal stages. The remaining flies have shortened lifespan, dying at around day 35 in comparison with wild type controls that have a lifespan of ~80 days. Third instar larvae exhibit impaired peristalsis and defective locomotion and adults have non-existent or severely impaired innate escape and climbing behaviours. Flies expressing TBPHScer\UAS.cDa under the control of Scer\GAL4EB1 show a reduction in walking activity over time, activity and total distance travelled compared with controls. However the mean walking velocity is comparable with controls. Severe gait abnormalities are seen when TBPHScer\UAS.cDa is expressed under the control of Scer\GAL4elav.PU.

Flies expressing TBPHScer\UAS.cDa under the control of Scer\GAL4elav.PU show no difference in either the amplitude or frequency of miniature excitatory junction potentials (mEJPs). Low-frequency stimulation showed no difference in evoked excitatory junction potential, and quantal content is similar to controls. No differences are seen following high-frequency stimulation.

The electroretinograms from 1 and 5 day old flies expressing TBPHScer\UAS.cDa under the control of Scer\GAL4GMR.PU in response to blue light pulses show a reduction in peak-peak amplitude compared to controls. No off/on transients are visible.

Expression of TBPHScer\UAS.cDa in the upper motor neurons under the control of Scer\GAL4EB1 does not significantly alter lifespan.

Flies expressing TBPHScer\UAS.cDa under the control of Scer\GAL4EB1 show axonal extensions and synaptic arborisations of ellipsoid body ring neurons. The phenotype worsens over time.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
TBPHScer\UAS.cDa
TBPHUAS.cDa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Diaper
Secondary FlyBase IDs
    References (3)