FB2026_02 , released June 18, 2026
Allele: Dmel\HtrA2IFD
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General Information
Symbol
Dmel\HtrA2IFD
Species
D. melanogaster
Name
FlyBase ID
FBal0287645
Feature type
allele
Associated gene
Dmel\HtrA2
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Yacobi-Sharon et al., 2013)
Progenitor genotype
Cytology
Description

A small in-frame deletion of 123 nucleotides (removes 41 amino acid residues, from T231 to V271). The deletion removes part of the serine protease domain, including the conserved catalytic S266 residue and another conserved S236 residue.

(Yacobi-Sharon and Arama, 2014.4.14)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:14,510,526..14,510,648 [-]
Comment:

A small in-frame deletion of 123 nt (from T231 to V271, 41 aa deleted).

(Yacobi-Sharon and Arama, 2014.4.14)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies heterozygous for HtrA2IFD display a 50%-60% reduction in levels of spermatogonial cyst death compared to wild type controls.

      HtrA2IFD/Df(3R)HtrA2Df1 transheterozygous flies display a 50%-60% reduction spermatogonial cyst death compared to wild type controls. The male flies produce only 2% of the number of progeny generated by wild-type, despite producing mature and motile sperm cells.

      HtrA2IFD/Df(3R)HtrA2Df2 transheterozygous flies display a 50%-60% reduction in spermatogonial cyst death compared to wild type controls.

      (Yacobi-Sharon et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      HtrA2IFD/Df(3R)HtrA2Df1 is partially rescued by HtrA2R-BAC

      (Yacobi-Sharon et al., 2013)

      HtrA2IFD/Df(3R)HtrA2Df1 is partially rescued by HtrA2+t1.97

      (Yacobi-Sharon et al., 2013)

      HtrA2IFD/Df(3R)HtrA2Df1 is partially rescued by HtrA2+t2.5

      (Yacobi-Sharon et al., 2013)

      HtrA2IFD/Df(3R)HtrA2Df1 is partially rescued by HtrA21.97.S266A

      (Yacobi-Sharon et al., 2013)

      HtrA2IFD/Df(3R)HtrA2Df1 is partially rescued by HtrA21.97.-IBM

      (Yacobi-Sharon et al., 2013)
      Comments

      Expression of two copies of HtrA2R-BAC partially rescues the reduction in spermatogonial cyst death seen in HtrA2IFD/Df(3R)HtrA2Df1 mutant flies. The amount of germline cell death is restored to approximately 80% of wild type levels.

      Expression of two copies of HtrA2+t2.5 partially rescues the spermatogonial cyst death seen in HtrA2IFD/Df(3R)HtrA2Df1 mutant flies. The amount of germline cell death is restored to approximately 80% of wild type levels.

      Expression of two copies of HtrA2+t1.97 partially rescues the spermatogonial cyst death seen in HtrA2IFD/Df(3R)HtrA2Df1 mutant flies. The amount of germline cell death is restored to approximately 80% of wild type levels. The defect in male sterility is also partially rescued.

      Expression of two copies of HtrA21.97.S266A partially rescues the spermatogonial cyst death seen in HtrA2IFD/Df(3R)HtrA2Df1 mutant flies. The amount of germline cell death is restored to approximately 60% of wild type levels. The male sterility defect is also slightly rescued.

      Expression of two copies of HtrA21.97.-IBM partially rescues the spermatogonial cyst death seen in HtrA2IFD/Df(3R)HtrA2Df1 mutant flies. The amount of germline cell death is restored to approximately 80% of wild type levels. The male sterility defect is also partially rescued, with rescue flies producing ~75% of the progeny seen in wild type.

      (Yacobi-Sharon et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      HtrA2IFD
      (Monteiro et al., 2023, Yacobi-Sharon and Arama, 2014.4.14)
      omiIFD
      (Yacobi-Sharon et al., 2013)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)