FlyBase Allele Report: Dmel\Utx[Δ]

General Information

Symbol

Dmel\Utx^Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0287837

Feature type

allele

Associated gene

Dmel\Utx

Associated Insertion(s)

TI{TI}Utx^Δ

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

Mutagen

ends-out gene targeting

(Copur and Müller, 2013)

Progenitor genotype

Associated Insertion(s)

TI{TI}Utx^Δ

Cytology

Description

Most of the Utx JmjC catalytic domain (nucleotides 2L:10273487..10275042 , release 5 genome) has been replaced by a mini-w marker, and a stop codon has been introduced in place of amino acid residue 503.

(Copur and Müller, 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:10,273,487..10,275,042 [-]

Comment:

Utx sequences (residues 503-952) were deleted with ends-out gene targeting and replaced with a mini-white gene and the AGC codon for Ser503 was replaced with an in-frame TAA termination codon after the His502 codon of Utx.

(Copur and Müller, 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

enhancer of variegation, with In(1)w^m4

(Shalaby et al., 2017)

fertile, with Utx^g.wt

(Copur and Müller, 2018)

lethal | germline clone | recessive | rescuable maternal effect

(Copur and Müller, 2018)

lethal | recessive

(Shalaby et al., 2017)

lethal - all die before end of prepupal stage | germline clone | rescuable maternal effect

(Copur and Müller, 2013)

majority die during larval stage | germline clone | recessive | rescuable maternal effect

(Copur and Müller, 2018)

majority die during larval stage | germline clone | rescuable maternal effect

(Copur and Müller, 2013)

short lived

(Copur and Müller, 2013)

short lived (with Df(2L)BSC143)

(Copur and Müller, 2013)

short lived | recessive

(Copur and Müller, 2018)

some die during third instar larval stage | germline clone | rescuable maternal effect

(Copur and Müller, 2013)

viable, with Utx^g.wt

(Copur and Müller, 2018)

Phenotype Manifest In

abdominal tergite 5 | male

(Copur and Müller, 2013)

haltere

(Copur and Müller, 2013)

wing | ectopic

(Copur and Müller, 2013)

Detailed Description

Statement

Reference

Utx^Δ/Utx^Δ adults derived from Utx^Δ/+ mothers die within 1-2 days post eclosion. When derived from Utx^Δ/+ mothers with homozygous Utx^Δ female germline clones, Utx^Δ/Utx^Δ flies die during larval stages.

Adults carrying the Utx^g.wt allele in a Utx^Δ/Utx^Δ background derived from Utx^Δ/+ mothers both with or without homozygous Utx^Δ female germline clones can be maintained as a healthy strain.

Adults carrying the Utx^g.cd allele in a Utx^Δ/Utx^Δ background derived from Utx^Δ/+ mothers appear morphologically indistinguishable from wild-types, but die within 1-2 days post eclosion.

(Copur and Müller, 2018)

Utx^Δ mutants are homozygous lethal. Utx^Δ enhances the variegated phenotype of the w locus in the In(1)w^m4 background but has no effect on the variegation in the Sb locus when combined with T(2;3)Sb^V.

(Shalaby et al., 2017)

The majority of homozygous Utx^Δ and Utx^Δ/Df(2L)BSC143 animals that are derived from Utx^Δ/+ females develop into adults that are morphologically indistinguishable from wild type, but die shortly after eclosion.

Homozygous animals derived from females carrying homozygous female germline clones arrest development before the pupal stage; 85% die before reaching the third larval instar and the remaining animals die during the third larval instar, with only 0.6% developing to form white prepupae, which than also die. This maternal effect lethality can be paternally rescued: animals derived from homozygous oocytes fertilised by wild-type sperm are viable and fertile. The majority of the rescued adults have normal morphology, but 30% of males show a patchy loss of pigmentation in the tergite of the fifth abdominal segment (representing a transformation into a more anterior segment) and 2.8% of individuals show transformation of parts of the haltere into wing tissue.

Homozygous clones in the eye-antennal imaginal disc do not show an overproliferation phenotype.

(Copur and Müller, 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Utx[+]/Utx^Δ is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Utx[+]/Utx^Δ is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

NOT Enhancer of

Statement

Reference

Utx[+]/Utx^Δ is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

NOT Suppressor of

Statement

Reference

Utx[+]/Utx^Δ is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

Utx[+]/Utx^Δ is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

NOT Enhancer of

Statement

Reference

Utx[+]/Utx^Δ is a non-enhancer of wing vein phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

NOT Suppressor of

Statement

Reference

Utx[+]/Utx^Δ is a non-suppressor of wing vein phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Combination with a single copy of Utx^Δ further enhances the massive eye overgrowth characteristic for adult flies expressing yki^{S168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF, while is does not significantly modify the disrupted wing vein phenotype of flies expressing smo^{5A.Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^C-765.

(Shalaby et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Utx^Δ is rescued by Utx^g.wt

(Copur and Müller, 2018)

Not rescued by

Utx^Δ is not rescued by Utx^g.cd

(Copur and Müller, 2018)

Comments

Utx^g.wt fully rescues adult mortality in Utx^Δ/Utx^Δ flies derived from Utx^Δ/+ mothers and larval lethality in Utx^Δ/Utx^Δ flies derived from Utx^Δ/+ mothers with homozygous Utx^Δ female germline clones. Conversely, Utx^g.cd fails to rescue mortality in both conditions.

(Copur and Müller, 2018)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

UTX^Δ

(Shalaby et al., 2017)

Utx^Δ

(Copur and Müller, 2018, Copur and Müller, 2013)

dUtxΔ

(Yung et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close