FlyBase Allele Report: Hsap\PRKN[WT.UAS]

General Information

Symbol

Hsap\PRKN^WT.UAS

Species

H. sapiens

Name

FlyBase ID

FBal0287974

Feature type

allele

Associated gene

Hsap\PRKN

Associated Insertion(s)

Carried in Construct

P{UAS-hParkin.WT}

Key Links

Transgenic product class

cDNA

(Wang et al., 2007)

wild_type

(Wang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Wang et al., 2007)

wild_type

(Wang et al., 2007)

Mutagen

in vitro construct

(Wang et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-hParkin.WT}

Cytology

Description

UASt regulates expression of wild type Hsap\PRKN cDNA.

(Wang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\PRKN

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Hsap\LRRK2^{G2019S.UAS.Tag:MYC}

(Ng et al., 2012, Ng et al., 2009)

ameliorates late onset Parkinson's disease

modeled by Hsap\ZNF746^DM.UAS

(Pirooznia et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant, with Scer\GAL4^Ddc.PL

(Wang et al., 2007)

partially lethal - majority die, with Scer\GAL4^Act5C.PI

(Pirooznia et al., 2020)

viable, with Scer\GAL4^Act5C.PI

(Pirooznia et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

Scer\GAL4^elav.PU-mediated expression of Hsap\PARK2^WT.Scer\UAS does not compromise the overall anatomical integrity of the adult brain.

PPL1 and PAM (as well as PAL, PPL2, PPM1/2 and PPM3) neurons remain intact in flies expressing Hsap\PARK2^WT.Scer\UAS via Scer\GAL4^Ddc.PL.

Scer\GAL4^elav.PU, Hsap\PARK2^WT.Scer\UAS fly brains do not exhibit loss of dopaminergic PPL1 neurons or serotonergic neurons.

Scer\GAL4^Ddc.PL, Hsap\PARK2^WT.Scer\UAS flies do not show a significant impairment in the ability in their climbing ability compared to controls.

The morphology of surviving PPL1 neurons in Scer\GAL4^Ddc.PL, Hsap\PARK2^WT.Scer\UAS flies exposed to rotenone is relatively uncompromised compared to controls. The number of PPL1, PPL2 and PAM neurons in Scer\GAL4^Ddc.PL, Hsap\PARK2^WT.Scer\UAS flies exposed to rotenone is considerably increased compared to controls. The climbing ability of Scer\GAL4^Ddc.PL, Hsap\PARK2^WT.Scer\UAS flies exposed to rotenone is considerably increased compared to controls.

The indirect flight muscles of Scer\GAL4^how-24B, Hsap\PARK2^WT.Scer\UAS flies do not show abnormal mitochondria - mitochondria are generally intact.

(Wang et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\PRKN^WT.UAS, Scer\GAL4^Act5C.PI is a suppressor | partially of increased mortality during development phenotype of Hsap\ZNF746^UAS.cDa, Scer\GAL4^Act5C.PI

(Pirooznia et al., 2020)

Hsap\PRKN^WT.UAS, Scer\GAL4^how-24B is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^how-24B

(Ng et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hsap\PRKN^WT.UAS, Scer\GAL4^how-24B is a suppressor of flight muscle cell | progressive phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^how-24B

(Ng et al., 2012)

Hsap\PRKN^WT.UAS, Scer\GAL4^how-24B is a suppressor of mitochondrion phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^how-24B

(Ng et al., 2012)

Hsap\PRKN^WT.UAS, Scer\GAL4^Ddc.PL is a suppressor of dopaminergic neuron phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2012)

Hsap\PRKN^WT.UAS, Scer\GAL4^Ddc.PL is a suppressor of mitochondrion phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2012)

Hsap\PRKN^WT.UAS, Scer\GAL4^Ddc.PL is a suppressor of dopaminergic PPM2 neuron | adult stage | chemical conditional phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2009)

Hsap\PRKN^WT.UAS, Scer\GAL4^Ddc.PL is a suppressor of dopaminergic PPM3 neuron | adult stage | chemical conditional phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2009)

Hsap\PRKN^WT.UAS, Scer\GAL4^Ddc.PL is a suppressor of dopaminergic PPM1 neuron | adult stage | progressive phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2009)

Hsap\PRKN^WT.UAS, Scer\GAL4^Ddc.PL is a suppressor of dopaminergic PPM3 neuron | adult stage | progressive phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2009)

Hsap\PRKN^WT.UAS/Scer\GAL4^Ddc.PL is a suppressor of dopaminergic PPL1 neuron | adult stage phenotype of park¹

(Wang et al., 2007)

Hsap\PRKN^WT.UAS/Scer\GAL4^Ddc.PL is a suppressor of adult dopaminergic PAM neuron | adult stage phenotype of park¹

(Wang et al., 2007)

Hsap\PRKN^WT.UAS/Scer\GAL4^how-24B is a suppressor of mitochondrion phenotype of park¹

(Wang et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\PARK2^WT.Scer\UAS suppresses the mitochondrial defects seen in the flight muscles when Hsap\LRRK2^{G2019S.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^how-24B. The climbing defects are also rescued.

Expression of Hsap\PARK2^WT.Scer\UAS suppresses the mitochondrial defects seen in dopaminergic neurons when Hsap\LRRK2^{G2019S.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^Ddc.PL.

(Ng et al., 2012)

The accelerated loss of DA neurons in the PPM 2/3 clusters of rotenone-treated flies expressing Hsap\LRRK2^{G2019S.Scer\UAS.T:Hsap\MYC} via Scer\GAL4^Ddc.PL is mitigated through co-expression of Hsap\PARK2^WT.Scer\UAS.

The significant degeneration of DA neurons in the PPM clusters 1 and 3 in 60 day old flies expressing Hsap\LRRK2^{G2019S.Scer\UAS.T:Hsap\MYC} via Scer\GAL4^Ddc.PL is mitigated through co-expression of Hsap\PARK2^WT.Scer\UAS.

(Ng et al., 2009)

Scer\GAL4^Ddc.PL-mediated expression of Hsap\PARK2^WT.Scer\UAS significantly protects against the degeneration of PPL1 and PAM dopaminergic neurons normally seen in 20-day old park¹ flies.

The mitochondrial defects seen in park¹ indirect flight muscles are significantly mitigated by Scer\GAL4^how-24B-mediated expression of Hsap\PARK2^WT.Scer\UAS.

(Wang et al., 2007)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\PARK2^WT.Scer\UAS

Hsap\PRKN^WT.Scer\UAS

Hsap\PRKN^WT.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

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