FB2026_02 , released June 18, 2026
Allele: Dmel\HtrA2NSO
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General Information
Symbol
Dmel\HtrA2NSO
Species
D. melanogaster
Name
FlyBase ID
FBal0288462
Feature type
allele
Associated gene
Dmel\HtrA2
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Yun et al., 2008)
Mutagen
ethyl methanesulfonate
(Yun et al., 2008)
Progenitor genotype
Cytology
Description

Nucleotide substitution: C?T.

(Yun et al., 2008)

Amino acid replacement: Q196term.

(Yun et al., 2008)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:14,510,821..14,510,821 [-]
Nucleotide change:

C14510821T

Reported nucleotide change:

C?T

Amino acid change:

Q196term | HtrA2-PA; Q196term | HtrA2-PB

Reported amino acid change:

Q196term

(Yun et al., 2008)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      HtrA2NSO/Df(3R)ED5644 mutant flies do not show any gross external defects. Females are fertile but 100% of males are sterile. In these males the seminal vesicles are empty and no motile sperm are observed.

      HtrA2NSO/Df(3R)ED5644 mutant males exhibit normal mitochondrial morphology in the testes. As in wild type, the spherical "onion stage" nebenkern in the spermatids appear smooth and display no vacuolisation and the usual two mitochondrial derivatives are seen during spermatid elongation (the "leaf blade" stage). Mitochondria continue to appear normal in post-individualisation spermatids, although individual spermatids are disorganised within the cyst and the investment cones are scattered, suggesting that the movement of these structures is asynchronous.

      HtrA2NSO/Df(3R)ED5644 mutants do not show any dopaminergic neuron loss in the brains of 40 day old flies at 25[o]C. Indirect flight muscles appear normal, even at 30 days, and EM analysis shows that mitochondrial integrity is as in wild type.

      100% of HtrA2V110E/HtrA2NSO mutant male flies are sterile. These flies display empty seminal vesicles and no motile sperm are observed.

      (Yun et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhancer of
      Statement
      Reference

      HtrA2NSO/Df(3R)ED5644 is a non-enhancer of spermatid phenotype of Pink15

      (Yun et al., 2008)

      HtrA2NSO/Df(3R)ED5644 is a non-enhancer of Nebenkern phenotype of Pink15

      (Yun et al., 2008)

      HtrA2NSO/Df(3R)ED5644 is a non-enhancer of mitochondrial derivative phenotype of Pink15

      (Yun et al., 2008)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A HtrA2NSO/Df(3R)ED5644 mutant background does not enhance the mitochondrial morphology defects seen in Pink15 mutant spermatids.

      (Yun et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      HtrA2NSO/Df(3R)ED5644 is rescued by HtrA2βTub85D.PY

      (Yun et al., 2008)

      HtrA2NSO/Df(3R)ED5644 is rescued by HtrA2G363S.βTub85D

      (Yun et al., 2008)
      Not rescued by

      HtrA2NSO/Df(3R)ED5644 is not rescued by HtrA2S266A.βTub85D

      (Yun et al., 2008)
      Comments

      Expression of HtrA2βTub85D.PY rescues the spermatogenesis defects seen in HtrA2NSO/Df(3R)ED5644 mutant males. Motile sperm are present in the seminal vesicles and the individualisation defects are suppressed.

      Expression of HtrA2S266A.βTub85D fails to rescue the sterility seen in HtrA2NSO/Df(3R)ED5644 mutant males. In these males the seminal vesicles are empty and no motile sperm are observed.

      Expression of HtrA2G363S.βTub85D rescues the sterility seen in HtrA2NSO/Df(3R)ED5644 mutant males. Motile sperm are observed in the seminal vesicles.

      (Yun et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Complementation data shows that the semi-lethality associated with HtrA2NSO homozygotes is due to a second site mutation.

      (Yun et al., 2008)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      HtrA2NSO
      omiNSO
      (Yun et al., 2008)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)