A 3683 base pair fragment from the flanking non-coding or intronic region of Lgr3 is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
GFP reconstitution across synaptic partners (GRASP) shows synaptic connections between Scer\GAL4Ptth.cMa-positive prothoracic gland innervating neurons and Ecol\lexAGMR19B09-positive dorsomedial neurosecretory cells.
Ecol\lexAGMR19B09 drives expression in around 12 neurons per hemisphere in the central larval brain as well as some neurons in the ventral nerve cord. GFP reconstitution across synaptic partners (GRASP) shows extensive connections to Scer\GAL4Ilp3.PC positive neurons.