A 3683 base pair fragment from the flanking non-coding or intronic region of Lgr3 is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
EcollexAGMR19B09 colocalizes with ScerGAL4VP16.Lgr3 in the median bundle of females, and has lower expression in males.
GFP reconstitution across synaptic partners (GRASP) shows synaptic connections between ScerGAL4Ptth.cMa-positive prothoracic gland innervating neurons and EcollexAGMR19B09-positive dorsomedial neurosecretory cells.
EcollexAGMR19B09 drives expression in around 12 neurons per hemisphere in the central larval brain as well as some neurons in the ventral nerve cord. GFP reconstitution across synaptic partners (GRASP) shows extensive connections to ScerGAL4Ilp3.PC positive neurons.
EcollexAGMR19B09 co-expresses with ScerGAL4Mz699 in a bilateral pair of neurons in the larval pars intercerebralis.