FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\tnMJO-348
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General Information
Symbol
Dmel\tnMJO-348
Species
D. melanogaster
Name
FlyBase ID
FBal0290989
Feature type
allele
Associated gene
Dmel\tn
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Domsch et al., 2013)
Progenitor genotype
tnMB03490
(Domsch et al., 2013)
Cytology
Description

Imprecise excision of Mi{ET1}tnMB03490 generates a 7.5kb deletion that removes the tn translation initiation site.

(Domsch et al., 2013)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      DOES NOT exacerbate  distal myopathy
      modeled by MhcK1728del
      (Dahl-Halvarsson et al., 2018)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      tnMJO-348 heterozygous adults does not induce any obvious sarcomere phenotype in indirect flight muscles.

      (Dahl-Halvarsson et al., 2018)

      Late stage embryos homozygous for tnMJO-348 or transheterozygous for tnMJO-348/Df(2R)Exel6068 do not show any muscles defects and hatch normally. However, abnormalities, such as body length reduction and reduced contraction are detected during the second larval instar and become more pronounced thereafter. By late third instar, the mutant larvae are significantly slimmer and shorter by ~10% than their control counterparts. The mutant larvae are flaccid and do not contract efficiently, thus compromising subsequent morphogenetic processes during pupation. As a consequence of these defects, tnMJO-348 pupae are longer and slimmer, exhibit an abnormal 'air pocket' in the posterior end, which is normally translocated anteriorly, and incomplete head eversion. Lethality occurs at early pupal stage.

      Loss of tn, in tnMJO-348 homozygotes or tnMJO-348/Df(2R)Exel6068 transheterozygotes, does not compromise muscle attachment. However, the mutant myofibers show signs of atrophy, being shorter and thinner suggesting some underlying sacromeric defects. Periodic F-actin striations are severely disrupted in mutant larvae when compared to control larvae.

      (Domsch et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      tnMJO-348 has lethal - all die before end of pupal stage phenotype, suppressible by sls[+]/slsj1D7

      (Domsch et al., 2013)

      tnMJO-348 has decreased body size phenotype, suppressible by sls[+]/slsj1D7

      (Domsch et al., 2013)
      NOT Enhancer of
      Statement
      Reference

      tn[+]/tnMJO-348 is a non-enhancer of abnormal locomotor behavior | adult stage | dominant phenotype of MhcK1728del

      (Dahl-Halvarsson et al., 2018)
      NOT Suppressor of
      Statement
      Reference

      tn[+]/tnMJO-348 is a non-suppressor of abnormal locomotor behavior | dominant | adult stage phenotype of MhcK1728del

      (Dahl-Halvarsson et al., 2018)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      tnMJO-348 has visceral muscle fiber phenotype, enhanceable by Actn14/Actn[+]

      (Domsch et al., 2013)

      tnMJO-348 has filamentous actin phenotype, enhanceable by Actn14/Actn[+]

      (Domsch et al., 2013)
      NOT Enhanced by
      Statement
      Reference

      tnMJO-348 has visceral muscle fiber phenotype, non-enhanceable by Actn14/Actn[+]

      (Domsch et al., 2013)

      tnMJO-348 has filamentous actin phenotype, non-enhanceable by Actn14/Actn[+]

      (Domsch et al., 2013)
      Suppressed by
      Statement
      Reference

      tnMJO-348 has visceral muscle fiber phenotype, suppressible by sls[+]/slsj1D7

      (Domsch et al., 2013)

      tnMJO-348 has filamentous actin phenotype, suppressible by sls[+]/slsj1D7

      (Domsch et al., 2013)
      NOT suppressed by
      Statement
      Reference

      tnMJO-348 has visceral muscle fiber phenotype, non-suppressible by mib21/mib2[+]

      (Domsch et al., 2013)

      tnMJO-348 has filamentous actin phenotype, non-suppressible by mib21/mib2[+]

      (Domsch et al., 2013)
      Enhancer of
      Statement
      Reference

      tn[+]/tnMJO-348 is an enhancer of indirect flight muscle cell | adult stage phenotype of MhcK1728del

      (Dahl-Halvarsson et al., 2018)

      tn[+]/tnMJO-348 is an enhancer of sarcomere | adult stage phenotype of MhcK1728del

      (Dahl-Halvarsson et al., 2018)
      Suppressor of
      Statement
      Reference

      tn[+]/tnMJO-348 is a suppressor of visceral muscle fiber phenotype of Mlp84BPΔ8-95

      (Domsch et al., 2013)

      tn[+]/tnMJO-348 is a suppressor of filamentous actin phenotype of Mlp84BPΔ8-95

      (Domsch et al., 2013)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The jump ability defects observed in MhcK1728del heterozygotes are not significantly affected by tnMJO-348 heterozygosity.

      (Dahl-Halvarsson et al., 2018)

      Pupae trans-heterozygous for tnMJO-348 and slsj1D7 undergo proper pupal morphogenesis and eclosion. Notably, mutant pupae homozygous for tnMJO-348 and heterozygous for slsj1D7 are slim and show a statistically significant increase in length when compared to homozygous tnMJO-348 pupae. This result indicates a suppression of the muscle contraction and morphogenetic defects that are associated with the tnMJO-348 mutant phenotype.

      Third instar larvae homozygous for Mlp84BPΔ8-95 and heterozygous for tnMJO-348 exhibit disrupted striations and myofiber tearing, although the defects are not as severe as in homozygous tnMJO-348 mutants. There are fewer adult 'escapers' for Mlp84BPΔ8-95 homozygous mutants in a tnMJO-348 heterozygous background than for Mlp84BPΔ8-95 homozygous mutants alone.

      Larvae heterozygous for Actn14 and tnMJO-348 display normal F-actin striations. By comparison, tnMJO-348 mutants in a Actn14 heterozygous background display more extensive disorganisation, indicating enhancement of the tn mutant phenotype.

      tnMJO-348 mutant larvae with reduced mib2 function (in a mib21 heterozygous background) do not exhibit enhanced defects when compared to tnMJO-348 single mutants.

      (Domsch et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      AbbaMJO0348
      (Dahl-Halvarsson et al., 2018)
      abbaMJO-348
      (Domsch et al., 2013)
      tnMJO-348
      Name Synonyms
      Secondary FlyBase IDs
        References (2)