Expression of Mi-2^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^da.G32 results in larval lethality; only 12% of animals survive beyond the third-larval instar.

Expression of Mi-2^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^da.G32 results in salivary gland polytene chromosomes being increased in volume compared to wild type. In fixed chromosome squashes the banding pattern is severely disrupted and it is often difficult to distinguish individual chromosome arms, while the banding pattern is clear in live analysis (suggesting that the chromosomes may be sensitive to mechanical stress during the squash procedure).

Animals carrying Mi-2^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^da.G32 and Scer\GAL80^ts.αTub84B show changes in polytene chromosome morphology within 10-12 hours after a shift from 18 to 29[o]C during the third larval instar (which inactivates the temperature sensitive Scer\GAL80^ts.αTub84B allele, allowing expression of Mi-2^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^da.G32). The salivary gland chromosomes show a significant increase in volume, which is caused by decondensation of the chromosomes as apposed to additional rounds of DNA replication.

The metaphase chromosomes in wing imaginal discs of third instar larvae expressing Mi-2^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^da.G32 at 29[o]C appear disorganised and less compact than normal.