FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\stepE173K.UASp.EGFP
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General Information
Symbol
Dmel\stepE173K.UASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0291707
Feature type
allele
Associated gene
Dmel\step
Associated Insertion(s)
Carried in Construct
P{UASp-step.E173K.EGFP}
Key Links
Transgenic product class
missense_variant
(Lee and Harris, 2013)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Lee and Harris, 2013)
Mutagen
in vitro construct
(Lee and Harris, 2013)
Progenitor genotype
Carried in construct
P{UASp-step.E173K.EGFP}
Cytology
Description

UASp regulatory sequences drive expression of a mutant form of step in which the glutamic acid residue at amino acid 173 has been replaced by lysine. The construct is tagged at the N-terminal end with EGFP.

(Lee and Harris, 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
step
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of stepE173K.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4ppk.PU leads to dorsal multidendritic ddaC neurons exhibiting no defects in the dendritic arborization at the white pupa stage, nor in pruning at 16h after puparium formation, as compared to controls.

      (Wang et al., 2017)

      Embryos derived from mothers expressing stepE173K.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 extend their furrow canals normally and synchronously.

      (Lee and Harris, 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4prd.RG1/stepE173K.UASp.EGFP fails to rescue stepKG09493/Df(2L)BSC150

      (West et al., 2017)
      Comments

      The expression of stepE173K.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4prd.RG1 fails to suppress the abnormal rosette formation in stepKG09493/Df(2L)BSC150 mutant epidermis at germband retraction.

      (West et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      stepE173K.Scer\UAS.P\T.T:Avic\GFP-EGFP
      stepE173K.UASp.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (6)