FlyBase Allele Report: Dmel\PpV[UASp.N.EGFP]

General Information

Symbol

Dmel\PpV^UASp.N.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0293990

Feature type

allele

Associated gene

Dmel\PpV

Associated Insertion(s)

Carried in Construct

P{UASp-PpV.EGFP.N}

Key Links

Transgenic product class

wild_type

(Lange, 2013.12.6)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lange, 2013.12.6)

Mutagen

in vitro construct

(Lange, 2013.12.6)

Progenitor genotype

Carried in construct

P{UASp-PpV.EGFP.N}

Cytology

Description

UASp sequences regulate expression of full-length PpV tagged at the N-terminus with EGFP.

(Lange, 2013.12.6)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

PpV

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.UAS, l(2)gl⁴

(Ma et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

PpV^UASp.N.EGFP, Scer\GAL4^FRT.Act5C is a suppressor | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2017)

fmt^UAS.cMa/PpV^UASp.N.EGFP, Scer\GAL4^FRT.Act5C is a suppressor | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2017)

fmt^UAS.cMa/PpV^UASp.N.EGFP, Scer\GAL4^GMR.PU is a suppressor | partially of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

fmt^UAS.cMa/PpV^UASp.N.EGFP, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

NOT Suppressor of

Statement

Reference

PpV^UASp.N.EGFP, Scer\GAL4^GMR.PU is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

PpV^UASp.N.EGFP, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

FBal0293990:, Scer\GAL4^GMR.PU, egr^UAS.cIa has eye phenotype, suppressible | partially by fmt^UAS.cMa/PpV^UASp.N.EGFP/PpV^UASp.N.EGFP, Scer\GAL4^GMR.PU

(Ma et al., 2017)

Suppressor of

Statement

Reference

fmt^UAS.cMa, PpV^UASp.N.EGFP, PpV^UASp.N.EGFP, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of FBal0293990:, Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

PpV^UASp.N.EGFP, Scer\GAL4^FRT.Act5C is a suppressor | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2017)

fmt^UAS.cMa/PpV^UASp.N.EGFP, Scer\GAL4^FRT.Act5C is a suppressor | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2017)

NOT Suppressor of

Statement

Reference

PpV^UASp.N.EGFP, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^{Scer\FRT.Act5C}>Ras85D^V12.Scer\UAS;l(2)gl⁴/l(2)gl⁴ are highly tumorigenic and have invasive capability and their overgrowth as well as invasiveness is significantly suppressed by co-expression of PpV^{Scer\UAS.P\T.N.T:Avic\GFP-EGFP} and to even greater extent when PpV^{Scer\UAS.P\T.N.T:Avic\GFP-EGFP} is expressed simultaneously with fmt^Scer\UAS.cMa in the mutant clones (however this effect is not due to increase in cell death).

The reduced eye size of Scer\GAL4^GMR.PU>egr^Scer\UAS.cIa adults is not suppressed by co-expressing either fmt^Scer\UAS.cMa or PpV^{Scer\UAS.P\T.N.T:Avic\GFP-EGFP} individually but simultaneous expression of both transgenes significantly ameliorates the small eye phenotype.

(Ma et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

53770

w^*; P{UASp-PpV.EGFP.N}3

53768

w^*; P{UASp-PpV.EGFP.N}2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

PpV^{Scer\UAS.P\T.N.T:Avic\GFP-EGFP}

PpV^UASp.N.EGFP

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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