FlyBase Allele Report: Dmel\fmt[UAS.cMa]

General Information

Symbol

Dmel\fmt^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ma

FlyBase ID

FBal0336301

Feature type

allele

Associated gene

Dmel\fmt

Associated Insertion(s)

Carried in Construct

P{UAS-fmt.M}

Key Links

Transgenic product class

wild_type

(Ma et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ma et al., 2017)

Mutagen

in vitro construct

(Ma et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-fmt.M}

Cytology

Description

UAS regulates expression of fmt.

(Ma et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

fmt

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.UAS, l(2)gl⁴

(Ma et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

fmt^UAS.cMa/Scer\GAL4^FRT.Act5C is a suppressor | partially | somatic clone of abnormal size | somatic clone | third instar larval stage phenotype of Scer\GAL4^FRT.Act5C, scrib¹

(Ma et al., 2017)

fmt^UAS.cMa, Scer\GAL4^FRT.Act5C is a suppressor | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2017)

fmt^UAS.cMa/PpV^UASp.N.EGFP, Scer\GAL4^FRT.Act5C is a suppressor | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2017)

fmt^UAS.cMa/PpV^UASp.N.EGFP, Scer\GAL4^GMR.PU is a suppressor | partially of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

fmt^UAS.cMa/PpV^UASp.N.EGFP, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

NOT Suppressor of

Statement

Reference

fmt^UAS.cMa/Scer\GAL4^GMR.PU is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

fmt^UAS.cMa/Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

fmt^UAS.cMa/Scer\GAL4^FRT.Act5C is a suppressor | partially | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Scer\GAL4^FRT.Act5C, scrib¹

(Ma et al., 2017)

fmt^UAS.cMa, Scer\GAL4^FRT.Act5C is a suppressor | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2017)

fmt^UAS.cMa/PpV^UASp.N.EGFP, Scer\GAL4^FRT.Act5C is a suppressor | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2017)

fmt^UAS.cMa, PpV^UASp.N.EGFP, PpV^UASp.N.EGFP, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of FBal0293990:, Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

NOT Suppressor of

Statement

Reference

fmt^UAS.cMa/Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^{Scer\FRT.Act5C}>Ras85D^V12.Scer\UAS;l(2)gl⁴/l(2)gl⁴ are highly tumorigenic and have invasive capability and their overgrowth as well as invasiveness is significantly suppressed by co-expression of fmt^Scer\UAS.cMa and to even greater extent when fmt^Scer\UAS.cMa is expressed simultaneously with PpV^{Scer\UAS.P\T.N.T:Avic\GFP-EGFP} in the mutant clones (however this effect is not due to increase in cell death).

The reduced eye size of Scer\GAL4^GMR.PU>egr^Scer\UAS.cIa adults is not suppressed by co-expressing either fmt^Scer\UAS.cMa or PpV^{Scer\UAS.P\T.N.T:Avic\GFP-EGFP} individually but simultaneous expression of both transgenes significantly ameliorates the small eye phenotype.

The small size of scrib¹ mutant MARCM clones that are recovered in third instar larval eye-antennal discs is significantly rescued if the clones also express fmt^Scer\UAS.cMa under the control of Scer\GAL4^{Scer\FRT.Act5C}.

(Ma et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

82143