FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\pufCA.HA.UAS.S
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General Information
Symbol
Dmel\pufCA.HA.UAS.S
Species
D. melanogaster
Name
FlyBase ID
FBal0294151
Feature type
allele
Associated gene
Dmel\puf
Associated Insertion(s)
Carried in Construct
P{UAS-puf.CA.HA-S}
Key Links
Transgenic product class
missense_variant
(Li et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Li et al., 2013)
Mutagen
in vitro construct
(Li et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-puf.CA.HA-S}
Cytology
Description

UASt regulates expression of a DNA encoding the short (RE) isoform of puf containing triple mutations: the catalytic cysteine (Cys2024) within the conserved cysteine box is replaced with alanine; and His2974 and Asp2302 within the histidine box are replaced with alanine.

(Li et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
puf
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4GMR.PU-mediated expression of pufCA.HA.Scer\UAS.S fails to elicit any detectable eye phenotype.

      Scer\GAL4Bx-MS1096-KE-mediated expression of pufCA.HA.Scer\UAS.S does not result in a detectable wing phenotype.

      (Li et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhancer of
      Statement
      Reference

      pufCA.HA.UAS.S, Scer\GAL4GMR.PU is a non-enhancer of eye phenotype of MycUAS.cZa, Scer\GAL4GMR.PU

      (Li et al., 2013)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      pufCA.HA.Scer\UAS.S
      pufCA.HA.UAS.S
      Name Synonyms
      Secondary FlyBase IDs
        References (1)