FlyBase Allele Report: Dmel\Hsc70-4[HMJ21529]

General Information

Symbol

Dmel\Hsc70-4^HMJ21529

Species

D. melanogaster

Name

FlyBase ID

FBal0294672

Feature type

allele

Associated gene

Dmel\Hsc70-4

Associated Insertion(s)

Carried in Construct

P{TRiP.HMJ21529}

Also Known As

UAS-Hsc70-4 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMJ21529}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMJ21529

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | P-stage, with Scer\GAL4^Mef2.PR

(Brooks et al., 2020)

abnormal body size | P-stage, with Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

female sterile, with Scer\GAL4^VP16.otu

(Yan and Perrimon, 2015)

lethal, with Scer\GAL4^nub-AC-62

(Yan and Perrimon, 2015)

lethal | female limited, with Scer\GAL4^dome-PG14

(Yan and Perrimon, 2015)

Phenotype Manifest In

anterior pupal spiracle | larval stage, with Scer\GAL4^Mef2.PR

(Brooks et al., 2020)

larval somatic muscle cell | larval stage, with Scer\GAL4^Mef2.PR

(Brooks et al., 2020)

Detailed Description

Statement

Reference

Expression of Hsc70-4^HMJ21529 under the control of Scer\GAL4^Mef2.PR leads to defective larval muscles with regions devoid of F-actin labeled myofibrillar material, elongated pupae (possibly due to failure to contract the body wall muscles during the larval to pupal transition) with defective spiracles and a failure to displace the abdominal air bubble.

(Brooks et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsc70-4^HMJ21529, Scer\GAL4^Mef2.PU has abnormal body size | P-stage phenotype, enhanceable by NdufA3^GD4003, Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

Enhancer of