FlyBase Allele Report: Dmel\Arf1[UAS.cKa]

General Information

Symbol

Dmel\Arf1^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct of Khadilkar

FlyBase ID

FBal0295788

Feature type

allele

Associated gene

Dmel\Arf1

Associated Insertion(s)

Carried in Construct

P{UAS-Arf1.K}

Key Links

Transgenic product class

cDNA

(Khadilkar et al., 2014)

wild_type

(Khadilkar et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Khadilkar et al., 2014)

wild_type

(Khadilkar et al., 2014)

Mutagen

in vitro construct

(Khadilkar et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-Arf1.K}

Cytology

Description

UAS regulatory sequences drive expression of a full-length Arf79F cDNA.

(Khadilkar et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Arf1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval crystal cell | third instar larval stage, with Scer\GAL4^{kn-col85-GAL4}

(Khadilkar et al., 2014)

embryonic/larval plasmatocyte | third instar larval stage, with Scer\GAL4^{kn-col85-GAL4}

(Khadilkar et al., 2014)

Detailed Description

Statement

Reference

Expression of Arf79F^Scer\UAS.cKa under the control of Scer\GAL4^e33C does not change circulating lamellocyte numbers in larvae.

(Khadilkar et al., 2017)

Expression of Arf79F^Scer\UAS.cKa in the niche (posterior signalling center) of the lymph gland primary lobe under the control of Scer\GAL4^{kn-col85-GAL4} has no effect on niche cell number. Prohemocyte differentiation into plasmatocytes and crystal cells is reduced.

(Khadilkar et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Arf1^UAS.cKa, Scer\GAL4^{kn-col85-GAL4} has embryonic/larval plasmatocyte | third instar larval stage phenotype, suppressible by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^{kn-col85-GAL4}

(Khadilkar et al., 2014)

Arf1^UAS.cKa, Scer\GAL4^{kn-col85-GAL4} has embryonic/larval crystal cell | third instar larval stage phenotype, suppressible by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^{kn-col85-GAL4}

(Khadilkar et al., 2014)

Suppressor of

Statement

Reference

Arf1^UAS.cKa, Scer\GAL4^Hml.Δ is a suppressor of embryonic/larval plasmatocyte | third instar larval stage phenotype of Pvr^GD14375, Scer\GAL4^Hml.Δ

(Khadilkar et al., 2014)

Arf1^UAS.cKa, Scer\GAL4^Hml.Δ is a suppressor of embryonic/larval crystal cell | third instar larval stage phenotype of Pvr^GD14375, Scer\GAL4^Hml.Δ

(Khadilkar et al., 2014)

NOT Suppressor of

Statement

Reference

Arf1^UAS.cKa, Scer\GAL4^{kn-col85-GAL4} is a non-suppressor of posterior signaling center of lymph gland primary lobe | third instar larval stage phenotype of Pten^UAS.cHa, Scer\GAL4^{kn-col85-GAL4}

(Khadilkar et al., 2014)

Other

Statement

Reference

Arf1^UAS.cKa, Pvr^GD14375, Scer\GAL4^Hml.Δ has posterior signaling center of lymph gland primary lobe | third instar larval stage phenotype

(Khadilkar et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Arf79F^Scer\UAS.cKa does not suppress the niche (posterior signalling center) cell loss seen in the lymph gland primary lobe when Pten^Scer\UAS.cHa is expressed under the control of Scer\GAL4^{kn-col85-GAL4}.

Co-expression of Arf79F^Scer\UAS.cKa and Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} under the control of Scer\GAL4^{kn-col85-GAL4} results in an enlarged niche (posterior signalling center) in the lymph gland primary lobe. Increased differentiation of prohemocytes into plasmatocytes and crystal cells is seen.

Co-expression of Arf79F^Scer\UAS.cKa and Pvr^GD14375 in the cortical zone under the control of Scer\GAL4^Hml.Δ has no effect on niche (posterior signalling center) cell number in the lymph gland primary lobe. The premature differentiation of hemocytes into plasmatocytes and crystal cells seen when Pvr^GD14375 is expressed alone is suppressed.

(Khadilkar et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^e33C/Arf1^UAS.cKa rescues Arf1^GD12522

(Khadilkar et al., 2017)

Comments

The increased number of melanized crystal cells following a heat-shock observed in third instar larvae expressing Arf79F^GD12522 under the control of Scer\GAL4^e33C is rescued by co-expression of Arf79F^Scer\UAS.cKa.

(Khadilkar et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Arf1^UAS.cKa

Arf79F^Scer\UAS.cKa

Arf79F^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct of Khadilkar

Secondary FlyBase IDs

References (2)

Report Sections

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