Expression of Arf79F^GD12522 does not impair the phagocytic ability of hemocytes either in adults (with the Scer\GAL4^Hml.PU driver) or in third instar larvae (using the Scer\GAL4^e33C driver), the larvae however show significantly increased number of melanized crystal cells following a heat-shock treatment (60[o]C for 10 min) compared to controls. Adult flies expressing Arf79F^GD12522 under the control of Scer\GAL4^e33C, Scer\GAL4^Hml.PU or Scer\GAL4^btl.PS display increased sensitivity (lower survival rate) upon infection with either Bacillus subtilis or Escherichia coli. Scer\GAL4^btl.PS-driven expression significantly lowers adult lifespan even without bacterial infection, no significant difference is observed with the Scer\GAL4^Hml.PU driver, as compared to controls.

Primary hemocytes from larvae expressing Arf79F^GD12522 under the control of Scer\GAL4^srp.PU show strongly reduced internalization of sSpi-GFP protein (spi encodes the principal ligand of the EGFR pathway) at high sSpi-GFP conditions (treatment with 30 ng/ml of sSpi-GFP) but not at low sSpi-GFP conditions (treatment with 4 ng/ml of sSpi-GFP).

The expression of Arf79F^GD12522 under the combined control of Scer\GAL4^ppk.PU and Dicer-2 transgene (for efficient RNAi) leads to dorsal multidendritic ddaC neurons exhibiting a significantly simplified dendritic tree at the white pupa stage and a delay in dendrite pruning at 16h-18h after puparium formation, but exhibiting no defects in the elimination of ddaF neurons by apoptosis at 16h after puparium formation, as compared to controls; at wandering third instar larval stage, these dorsal multidendritic ddaC neurons also exhibit defects in the Golgi apparatus, as shown by the virtual absence of GalT and ManII punctate structures, and defects in the formation of secretory vesicles, as shown by the virtual absence of Sec15 punctate structures, as compared to controls.

Expression of Arf79F^GD12522 in the entire third instar larval lymph gland under the control of Scer\GAL4^e33C results in hyperproliferation of the secondary lobe. Phenotypes are also seen in the primary lobe; a significantly larger population of cortical zone cells are found to be in S phase, and more cells are mitotically active cells compared to controls. The cortical zone is expanded, indicating premature differentiation of prohemocytes into plasmatocytes and crystal cells. The number of cells in the niche (posterior signalling center) is also reduced. Total hemocyte number in circulation is increased from the second larval instar stage onwards.

Expression of Arf79F^GD12522 in the niche (posterior signalling center) of the lymph gland primary lobe under the control of Scer\GAL4^{kn-col85-GAL4} results in complete loss of niche cells from the first instar larval stage onwards, with increased differentiation into plasmatocytes and crystal cells. No change in circulating hemocyte number is seen.

Expression of Arf79F^GD12522 in the medullary zone of the lymph gland primary lobe, under the control of Scer\GAL4^dome-PG14, has no effect on the number of cells in the niche (posterior signalling center). Increased differentiation of prohemocytes into plasmatocytes and crystal cells is seen but there is no change in circulating hemocyte number.

Expression of Arf79F^GD12522 in the cortical zone of the lymph gland primary lobe under the control of Scer\GAL4^Pxn.PS causes a moderate reduction in the number of niche (posterior signalling center) cells and increased differentiation of prohemocytes into plasmatocytes and crystal cells. Expressing Arf79F^GD12522 under the control of Scer\GAL4^Pxn.PS perturbs the hemocyte balance, resulting in fewer plasmatocytes and an increased number of lamellocytes in circulation.

Expression of Arf79F^GD12522 in the cortical zone of the lymph gland primary lobe under the control of Scer\GAL4^Hml.Δ causes a moderate reduction in the number of niche (posterior signalling center) cells and increased differentiation of prohemocytes into plasmatocytes and crystal cells.

Expression of Arf79F^GD12522 in circulating hemocytes (but not in lymph gland hemocytes) under the control of Scer\GAL4^gcm.PU results in increased differentiation of plasmatocytes and crystal cells in the lymph gland. There is no change in the number of niche (posterior signalling center) cells in the lymph gland primary lobe. The total and differential count of circulating hemocytes is increased.

Expression of Arf79F^GD12522 under the control of Scer\GAL4^Hml.Δ results in endocytic trafficking defects during larval haematopoesis. NICD is trapped in Hrs positive endosomes.

Adults expressing Arf79F^GD12522 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

Arf1^GD12522, Scer\GAL4^ppk.PU has abnormal neuroanatomy | pupal stage phenotype, suppressible | partially by Nrg^GL00656, Scer\GAL4^ppk.PU

Arf1^GD12522, Scer\GAL4^ppk.PU has abnormal neuroanatomy | pupal stage phenotype, suppressible | partially by Nrg^HMS01638, Scer\GAL4^ppk.PU

Arf1^GD12522, Scer\GAL4^ppk.PU has dendritic tree | pupal stage phenotype, suppressible | partially by Nrg^GL00656, Scer\GAL4^ppk.PU

Arf1^GD12522, Scer\GAL4^ppk.PU has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible | partially by Nrg^HMS01638, Scer\GAL4^ppk.PU

Arf1^GD12522, Scer\GAL4^ppk.PU has dendritic tree | pupal stage phenotype, suppressible | partially by Nrg^HMS01638, Scer\GAL4^ppk.PU

Arf1^GD12522, Scer\GAL4^ppk.PU has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible | partially by Nrg^GL00656, Scer\GAL4^ppk.PU

Arf1^GD12522, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)} has posterior signaling center of lymph gland primary lobe | third instar larval stage phenotype, suppressible | partially by Pten^UAS.cHa/Scer\GAL4^{kn-col85-GAL4}

Arf1^GD12522, Scer\GAL4^{kn-col85-GAL4} has embryonic/larval plasmatocyte | third instar larval stage phenotype, suppressible | partially by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^{kn-col85-GAL4}

Arf1^GD12522, Scer\GAL4^{kn-col85-GAL4} has embryonic/larval crystal cell | third instar larval stage phenotype, suppressible | partially by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^{kn-col85-GAL4}

Arf1^GD12522, Scer\GAL4^Hml.Δ has posterior signaling center of lymph gland primary lobe | third instar larval stage phenotype, suppressible by Pvr^UAS.cDa, Scer\GAL4^Hml.Δ

Arf1^GD12522, Scer\GAL4^{kn-col85-GAL4} has posterior signaling center of lymph gland primary lobe | third instar larval stage phenotype, non-suppressible by Pten^UAS.cHa, Scer\GAL4^{kn-col85-GAL4}

Arf1^GD12522, Scer\GAL4^{kn-col85-GAL4} has embryonic/larval plasmatocyte | third instar larval stage phenotype, non-suppressible by Pten^UAS.cHa, Scer\GAL4^{kn-col85-GAL4}

Arf1^GD12522, Scer\GAL4^{kn-col85-GAL4} has embryonic/larval crystal cell | third instar larval stage phenotype, non-suppressible by Pten^UAS.cHa, Scer\GAL4^{kn-col85-GAL4}

Arf1^GD12522, Scer\GAL4^Hml.Δ has embryonic/larval plasmatocyte | third instar larval stage phenotype, non-suppressible by Pvr^UAS.cDa, Scer\GAL4^Hml.Δ

Arf1^GD12522, Scer\GAL4^Hml.Δ has embryonic/larval crystal cell | third instar larval stage phenotype, non-suppressible by Pvr^UAS.cDa, Scer\GAL4^Hml.Δ