FlyBase Allele Report: Dmel\Septin2[1]

General Information

Symbol

Dmel\Septin2¹

Species

D. melanogaster

Name

FlyBase ID

FBal0295818

Feature type

allele

Associated gene

Dmel\Septin2

Associated Insertion(s)

Carried in Construct

Also Known As

Sep2¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(O'Neill and Clark, 2013)

Progenitor genotype

P{EPgy2}Septin2^EY05856

(O'Neill and Clark, 2013)

Cytology

Description

Excision of P{EPgy2}Sep2^EY05856 resulting in deletion of the 5' half of Sep2, corresponding to Release 5 coordinates 3R:16412954..16414040 .

(O'Neill and Clark, 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:20,587,232..20,588,318 [-]

Comment:

1087 bp deleiton resulting from the imprecise excision of P{EPgy2}Septin2^EY05856 (removes 3R:16412954..16414040 in R5 coordinates).

(O'Neill and Clark, 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg | maternal effect

(O'Neill and Clark, 2013)

nurse cell

(O'Neill and Clark, 2013)

Detailed Description

Statement

Reference

Homozygous adults lay eggs with a shorter anterior-posterior axis than wild type.

Sibling crosses between homozygotes either produce no eggs, or eggs that don't hatch. Crosses between homozygous females and wild type males result in a few viable eggs, but eggs are not produced or don't hatch in the majority of cases. Crosses between homozygous males and wild type females result in a viable eggs in 50% of cases, and unhatched eggs in the other 50%.

About half of Sep2¹/Sep2² transheterozygotes die as pharates.

Only 53% of Sep2¹ egg chambers have the wild type number of nurse cell nuclei (15); the remaining chambers have either more or fewer, ranging from 3 to 57.

(O'Neill and Clark, 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Septin2¹ has abnormal cell cycle phenotype, non-suppressible by Scer\GAL4^Act5C.PI/Septin5^UASp.cOa

(O'Neill and Clark, 2013)

Other

Statement

Reference

Septin2²/Septin2¹, Septin5²/Septin5¹ has lethal - all die during prepupal stage phenotype

(O'Neill and Clark, 2013)

Septin2¹, Septin5² has abnormal cell cycle | larval stage phenotype

(O'Neill and Clark, 2013)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Septin2¹ has nurse cell phenotype, non-suppressible by Scer\GAL4^Act5C.PI/Septin5^UASp.cOa

(O'Neill and Clark, 2013)

Other

Statement

Reference

Septin2²/Septin2¹, Septin5²/Septin5¹ has imaginal disc | third instar larval stage phenotype

(O'Neill and Clark, 2013)

Septin2¹, Septin5² has neuroblast | larval stage phenotype

(O'Neill and Clark, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^Act5C.PI-mediated expression of Sep5^{Scer\UAS.P\T.cOa} only provides a weak rescue of the abnormal nurse cell number of Sep2¹ egg chambers in some cases.

Sep2¹/Sep2², Sep5¹/Sep5² animals arrest as prepupae. Wandering third instars of this genotype completely lack imaginal discs.

A low percentage of Sep5², Sep2¹ larval neuroblasts have tetraploid nuclei, compared to 0% in wild type.

(O'Neill and Clark, 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Septin2¹ is rescued by Septin2^UASp.cOa/Scer\GAL4^Act5C.PI

(O'Neill and Clark, 2013)

Partially rescued by

Septin2¹ is partially rescued by Septin2^UASp.cOa/Scer\GAL4^{VP16.mat.αTub67C}

(O'Neill and Clark, 2013)

Comments

Scer\GAL4^Act5C.PI-mediated expression of Sep2^{Scer\UAS.P\T.cOa} rescues the abnormal nurse cell number seen in Sep2¹ egg chambers.

Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}-mediated expression of Sep2^{Scer\UAS.P\T.cOa} partially rescues the abnormal nurse cell number seen in Sep2¹ egg chambers.

(O'Neill and Clark, 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

91002

w^*; Septin2¹/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Sep2¹

(Deb and Hasan, 2019, O'Neill and Clark, 2013)

Septin2¹

(Gabbert et al., 2023)

ΔdSEPT2¹

(Deb and Hasan, 2019)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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