FlyBase Allele Report: Dmel\ecd[RNAi.UAS.WIZ]

General Information

Symbol

Dmel\ecd^RNAi.UAS.WIZ

Species

D. melanogaster

Name

FlyBase ID

FBal0296202

Feature type

allele

Associated gene

Dmel\ecd

Associated Insertion(s)

Carried in Construct

P{UAS-ecd.RNAi.WIZ}

Also Known As

UAS-ecd^RNAi

Key Links

Transgenic product class

RNAi_reagent

(Claudius et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Claudius et al., 2014)

Mutagen

in vitro construct

(Claudius et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-ecd.RNAi.WIZ}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a 497bp cDNA fragment of the ecd gene.

(Claudius et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

ecd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult posterior midgut epithelium | heat sensitive, with Scer\GAL4^esg.PU

(Erkelenz et al., 2021)

anterior crossvein, with Scer\GAL4^dpp.PU

(Claudius et al., 2014)

first posterior wing cell, with Scer\GAL4^dpp.PU

(Claudius et al., 2014)

prothoracic gland, with Scer\GAL4^dpp.PU

(Claudius et al., 2014)

wing disc, with Scer\GAL4^dpp.PU

(Claudius et al., 2014)

Detailed Description

Statement

Reference

The expression of ecd^{dsRNA.UAS.WIZ} for 20 days from day 3 after eclosion, under the combined control of Scer\GAL4^esg.PU and Gal80[ts], leads to fewer intestine progenitors (ISCs and enteroblasts) in the adult posterior midgut, as compared to controls.

(Erkelenz et al., 2021)

Clones in the wing disc expressing ecd^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^Act5C.PI are eliminated within 72 hours after induction of ecd^{dsRNA.Scer\UAS.WIZ} expression.

Expression of ecd^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^dpp.PU results in the appearance of apoptotic cells in the wing imaginal disc. This gives rise to adults with wings that have a reduction in the intervein region corresponding to the Scer\GAL4^dpp.PU expression domain and which lack the anterior crossvein.

Animals expressing ecd^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^phm.PO reach the third larval instar but cannot pupate. The mutant larvae are smaller than controls at 6 days after egg laying. The prothoracic gland cells are reduced in size and have smaller nuclei than normal in the mutant larvae.

(Claudius et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^dpp.PU, ecd^RNAi.UAS.WIZ has increased cell death phenotype, suppressible by Hsap\ECD^UAS.Tag:FLAG, Scer\GAL4^dpp.PU

(Claudius et al., 2014)

Scer\GAL4^dpp.PU, ecd^RNAi.UAS.WIZ has visible phenotype, suppressible by Hsap\ECD^UAS.Tag:FLAG, Scer\GAL4^dpp.PU

(Claudius et al., 2014)

Scer\GAL4^phtm.PO, ecd^RNAi.UAS.WIZ has lethal - all die before end of larval stage phenotype, suppressible by Hsap\ECD^UAS.Tag:FLAG, Scer\GAL4^phtm.PO

(Claudius et al., 2014)

Scer\GAL4^phtm.PO, ecd^RNAi.UAS.WIZ has decreased body size phenotype, suppressible by Hsap\ECD^UAS.Tag:FLAG, Scer\GAL4^phtm.PO

(Claudius et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^dpp.PU, ecd^RNAi.UAS.WIZ has wing disc phenotype, suppressible by Hsap\ECD^UAS.Tag:FLAG, Scer\GAL4^dpp.PU

(Claudius et al., 2014)

Scer\GAL4^dpp.PU, ecd^RNAi.UAS.WIZ has anterior crossvein phenotype, suppressible by Hsap\ECD^UAS.Tag:FLAG, Scer\GAL4^dpp.PU

(Claudius et al., 2014)

Scer\GAL4^dpp.PU, ecd^RNAi.UAS.WIZ has first posterior wing cell phenotype, suppressible by Hsap\ECD^UAS.Tag:FLAG, Scer\GAL4^dpp.PU

(Claudius et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\ECD^{Scer\UAS.T:Zzzz\FLAG} suppresses the appearance of apoptotic cells in the wing imaginal discs of animals expressing ecd^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^dpp.PU and also suppresses the wing defects seen in these animals at the adult stage.

Co-expression of Hsap\ECD^{Scer\UAS.T:Zzzz\FLAG} restores normal growth and development to adults of animals expressing ecd^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^phm.PO.

(Claudius et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ecd^RNAi.UAS.WIZ

ecd^{dsRNA.Scer\UAS.WIZ}

ecd^{dsRNA.UAS.WIZ}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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