Insertion of a Mi{Hto-WP} element within Actβ ( 4:107 ,986 (+) -Release 6). The hostile takeover (hto) exon in the Mi{Hto-WP} element splices to the downstream sv exon 2. This results in a fusion protein being expressed under the control of Scer\UAS regulatory sequences in the Mi{Hto-WP} element. The fusion protein consists of 3 copies of the T:Zzzz\FLAG tag, the mCherry form of Disc\RFP, a 9 amino acid linker sequence translated from the sv 5'UTR exon (ELIHFVESL) and the coding sequence of sv.
The Mi{Hto-WP}ActβOMD insertion lies within the Actβ transcription unit. The insertion targets the downstream sv gene (a protein-trap fusion protein can be overexpressed from the Scer\UAS sequences within the Mi{Hto-WP} element in the presence of a Scer\GAL4 driver).