FlyBase Allele Report: Dmel\Uvrag[LL03097]

General Information

Symbol

Dmel\Uvrag^LL03097

Species

D. melanogaster

Name

FlyBase ID

FBal0296365

Feature type

allele

Associated gene

Dmel\Uvrag

Associated Insertion(s)

PBac{SAstopDsRed}Uvrag^LL03097

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

PBac{SAstopDsRed}Uvrag^LL03097

Cytology

Description

Insertion in the first coding exon.

(Issman-Zecharya and Schuldiner, 2014)

Allele components

Component

Use(s)

Inserted element

PBac{SAstopDsRed}

gene trap

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:13,287,006..13,287,006

(Issman-Zecharya and Schuldiner, 2014, Takáts et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of colorectal cancer

CEA

(Nagy et al., 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color | somatic clone

(Takáts et al., 2014)

abnormal neuroanatomy | adult stage | somatic clone

(Meltzer et al., 2019, Issman-Zecharya and Schuldiner, 2014)

abnormal neuroanatomy | pupal stage | somatic clone

(Issman-Zecharya and Schuldiner, 2014)

increased cell number | adult stage | somatic clone

(Nagy et al., 2016)

lethal - all die before end of larval stage (with Uvrag^B21)

(Takáts et al., 2014)

Phenotype Manifest In

adult midgut epithelium | somatic clone

(Nagy et al., 2016)

autolysosome | wandering third instar larval stage | nutrition conditional

(Takáts et al., 2014)

axon | adult stage

(Meltzer et al., 2019)

gamma Kenyon cell | adult stage | somatic clone

(Meltzer et al., 2019, Issman-Zecharya and Schuldiner, 2014)

gamma Kenyon cell | pupal stage | somatic clone

(Issman-Zecharya and Schuldiner, 2014)

Detailed Description

Statement

Reference

Uvrag^LL03097 mushroom body gamma neuron clones frequently show pruning defects.

(Meltzer et al., 2019)

The number of autophagic structures in the Uvrag^LL03097 mutant somatic clones of fat body cells of starved third instar larvae is comparable to surrounding control cells.

(Hegedűs et al., 2016)

Uvrag^LL03097 homozygous clones within a mosaic adult midgut epithelium show a severe increase in cell number compared to control clones.

(Nagy et al., 2016)

Homozygous mushroom body gamma neurons show a moderate but robust axon and dendrite pruning defect, which is evident at 18 hours after puparium formation and persists to the adult stage.

(Issman-Zecharya and Schuldiner, 2014)

Autolysosome formation appears normal in Uvrag^LL03097 mutant fat body cell clones in starved wandering third instar larvae. No accumulation of autophagosomes is seen in Uvrag^LL03097/Df(2L)ED784 mutant fat body cells and lysosomal shape appears normal. However unlike controls more advanced stages of degradation are never seen in Uvrag^LL03097 mutant lysosomes.

Adult flies containing eyes entirely composed of Uvrag^LL03097 mutant cells (generated using the GMR-hid method) are lighter in color compared to controls.

(Takáts et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Uvrag^LL03097 has abnormal neuroanatomy | somatic clone phenotype, enhanceable by Scer\GAL4^Tab2-201Y/ptc^UAS.cJa

(Issman-Zecharya and Schuldiner, 2014)

Uvrag^LL03097 has abnormal neuroanatomy | somatic clone phenotype, enhanceable by ptc^UAS.cJa/Scer\GAL4^GMR71G10

(Issman-Zecharya and Schuldiner, 2014)

NOT suppressed by

Statement

Reference

Uvrag^LL03097 has abnormal neuroanatomy | somatic clone phenotype, non-suppressible by Scer\GAL4^Tab2-201Y/smo^CA.UAS.cUa

(Issman-Zecharya and Schuldiner, 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Uvrag^LL03097 has gamma Kenyon cell | somatic clone phenotype, enhanceable by Scer\GAL4^Tab2-201Y/ptc^UAS.cJa

(Issman-Zecharya and Schuldiner, 2014)

Uvrag^LL03097 has gamma Kenyon cell | somatic clone phenotype, enhanceable by ptc^UAS.cJa/Scer\GAL4^GMR71G10

(Issman-Zecharya and Schuldiner, 2014)

NOT suppressed by

Statement

Reference

Uvrag^LL03097 has gamma Kenyon cell | somatic clone phenotype, non-suppressible by Scer\GAL4^Tab2-201Y/smo^CA.UAS.cUa

(Issman-Zecharya and Schuldiner, 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ptc^Scer\UAS.cJa under the control of either Scer\GAL4^Tab2-201Y or Scer\GAL4^GMR71G10 enhances the pruning defect seen in Uvrag^LL03097 mushroom body gamma neuron clones.

Expression of smo^{CA.Scer\UAS.cUa} under the control of Scer\GAL4^Tab2-201Y does not suppress the pruning defect seen in Uvrag^LL03097 mushroom body gamma neuron clones.

(Issman-Zecharya and Schuldiner, 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Uvrag^LL03097 is rescued by Scer\GAL4^NP5130/Uvrag^UAS.Tag:FLAG

(Nagy et al., 2016)

Uvrag^LL03097 is rescued by Scer\GAL4^Tab2-201Y/Uvrag^{UAS.cIa.Tag:FLAG}

(Issman-Zecharya and Schuldiner, 2014)

Comments

The increased cell number observed in Uvrag^LL03097 adult midgut clones is suppressed by the expression of Uvrag^UAS.Tag:FLAG under the control of Scer\GAL4^esg-NP5130.

(Nagy et al., 2016)

Expression of Uvrag^{Scer\UAS.cIa.T:Zzzz\FLAG} under the control of Scer\GAL4^Tab2-201Y completely rescues the pruning defect seen in Uvrag^LL03097 mushroom body gamma neuron clones.

(Issman-Zecharya and Schuldiner, 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

141002

y^* w^*; PBac{SAstopDsRed}Uvrag^LL03097 P{neoFRT}40A P{FRT(w^hs)}G13 cn¹ bw¹/CyO, S^* bw¹

Notes on Origin

Discoverer

Comments

Precise excision of the PBac{SAstopDsRed}Uvrag^LL03097 insertion reverts the mushroom body gamma neuron pruning defect.

(Issman-Zecharya and Schuldiner, 2014)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

UVRAG^LL03097

(Meltzer et al., 2019, Hegedűs et al., 2016, Issman-Zecharya and Schuldiner, 2014)

UVRAG^LL

(Hegedűs et al., 2016)

UVRAG[LL]

(Takáts et al., 2014)

Uvrag^LL03097

(Nagy et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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