UAS regulatory sequences drive expression of a full-length Med open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.
Expression of MedScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in male flies with severe wing size and patterning defects.
Expression of MedScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in female flies with weak wing size and patterning defects.
MedUAS.ORF.GW.Tag:HA, Scer\GAL4ppk.PU is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\ATXN3tr.Q70.cc0.UAS.Tag:HA,Tag:MYC, Scer\GAL4ppk.PU
MedUAS.ORF.GW.Tag:HA, Scer\GAL4ppk.PU is a suppressor | partially of larval multidendritic class IV neuron | third instar larval stage phenotype of Hsap\ATXN3tr.Q70.cc0.UAS.Tag:HA,Tag:MYC, Scer\GAL4ppk.PU