FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Tgs1UAS.ORF.GW.Tag:HA
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General Information
Symbol
Dmel\Tgs1UAS.ORF.GW.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0298502
Feature type
allele
Associated gene
Dmel\Tgs1
Associated Insertion(s)
Carried in Construct
M{UAS-Tgs1.ORF.3xHA.GW}
Key Links
Transgenic product class
wild_type
(Bischof and FlyORF project members, 2014.6.20)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Bischof and FlyORF project members, 2014.6.20)
Mutagen
in vitro construct
(Bischof and FlyORF project members, 2014.6.20)
Progenitor genotype
Carried in construct
M{UAS-Tgs1.ORF.3xHA.GW}
Cytology
Description

UAS regulatory sequences drive expression of a full-length Tgs1 open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

(Bischof and FlyORF project members, 2014.6.20)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Tgs1
Tagged with
Tag:HA
Also carries
FRT2
FRT5
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Tgs1Scer\UAS.ORF.GW.T:Ivir\HA1 under the control of any of the following: Scer\GAL4Mef2.PR, Scer\GAL4how-24B, Scer\GAL4da.PU and Scer\GAL4c179 leads to complete lethality or reduced adult viability at 25[o]C and 29[o]C with more severe effects being observed at the higher temperature. Expression driven by Scer\GAL4G7 or Scer\GAL4elav.PU or Scer\GAL4RapGAP1-OK6 does not significantly decrease adult viability at either of the two temperatures.

      Expression of Tgs1Scer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4how-24B results in significant decrease in the rate of body wall muscle contractions in third instar larvae and increased length (length/width axial ratio) of pupae compared to controls.

      (Borg et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL41032.hx/Tgs1UAS.ORF.GW.Tag:HA rescues Tgs1GD14932

      (Borg et al., 2016)
      Comments

      Co-expression of Tgs1Scer\UAS.ORF.GW.T:Ivir\HA1 rescues the lethality of flies expressing Tgs1GD14932 under the control of Scer\GAL41032.hx.

      (Borg et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Tgs1Scer\UAS.ORF.GW.T:Ivir\HA1
      Tgs1UAS.ORF.GW.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (3)