FlyBase Allele Report: Dmel\Tgs1[GD14932]

General Information

Symbol

Dmel\Tgs1^GD14932

Species

D. melanogaster

Name

FlyBase ID

FBal0202802

Feature type

allele

Associated gene

Dmel\Tgs1

Associated Insertion(s)

Carried in Construct

P{GD14932}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14932}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14932

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates childhood spinal muscular atrophy

modeled by Smn^HMC03832

(Maccallini et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Dcr-2^UAS.cUa, Scer\GAL4^elav.PU

(Borg et al., 2016)

lethal, with Dcr-2^UAS.cUa, Scer\GAL4^RapGAP1-OK6

(Borg et al., 2016)

lethal, with Scer\GAL4^1032.hx

(Borg et al., 2016)

lethal, with Scer\GAL4^c179

(Borg et al., 2016)

lethal, with Scer\GAL4^da.PU

(Borg et al., 2016)

lethal, with Scer\GAL4^G7

(Borg et al., 2016)

lethal, with Scer\GAL4^how-24B

(Borg et al., 2016)

lethal, with Scer\GAL4^RapGAP1-OK6

(Borg et al., 2016)

partially lethal - majority die, with Dcr-2^UAS.cUa, Scer\GAL4^elav.PU

(Borg et al., 2016)

partially lethal - majority die, with Dcr-2^UAS.cUa, Scer\GAL4^Mef2.PR

(Borg et al., 2016)

partially lethal - majority die, with Dcr-2^UAS.cUa, Scer\GAL4^RapGAP1-OK6

(Borg et al., 2016)

partially lethal - majority die, with Scer\GAL4^elav.PU

(Borg et al., 2016)

partially lethal - majority die, with Scer\GAL4^G7

(Borg et al., 2016)

partially lethal - majority die, with Scer\GAL4^insc-Mz1407

(Borg et al., 2016)

partially lethal - majority die, with Scer\GAL4^RapGAP1-OK6

(Borg et al., 2016)

partially lethal - majority live, with Dcr-2^UAS.cUa, Scer\GAL4^Mef2.PR

(Borg et al., 2016)

partially lethal - majority live, with Scer\GAL4^elav.PU

(Borg et al., 2016)

partially lethal - majority live, with Scer\GAL4^insc-Mz1407

(Borg et al., 2016)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Borg et al., 2016)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Tgs1^GD14932 under the control of Scer\GAL4^Mef2.PR, Scer\GAL4^elav.PU or Dcr-2^Scer\UAS.cUa (all with or without Dcr-2^Scer\UAS.cUa to enhance RNAi efficiency) or any of the following: Scer\GAL4^c179, Scer\GAL4^how-24B, Scer\GAL4^G7, Scer\GAL4^insc-Mz1407, Scer\GAL4^da.PU or Scer\GAL4^1032.hx (all without Dcr-2^Scer\UAS.cUa) reduces adult viability to a varying degree (up to a point of complete lethality in some cases) at both 25[o] and 29[o]C with more severe effects being observed at the higher temperature.

(Borg et al., 2016)

Adults expressing Tgs1^GD14932 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development