FlyBase Allele Report: Dmel\Der-1[UAS.cLa]

General Information

Symbol

Dmel\Der-1^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Liang

FlyBase ID

FBal0299078

Feature type

allele

Associated gene

Dmel\Der-1

Associated Insertion(s)

Carried in Construct

P{UAS-Der-1.L}

Key Links

Transgenic product class

cDNA

(Liang et al., 2014)

wild_type

(Liang et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Liang et al., 2014)

wild_type

(Liang et al., 2014)

Mutagen

in vitro construct

(Liang et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-Der-1.L}

Cytology

Description

UAS regulatory sequences drive expression of a Der-1 cDNA (GH08782).

(Liang et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Der-1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates inclusion body myopathy with Paget disease of bone and frontotemporal dementia

modeled by TER94^R152H.UAS

(Liang et al., 2014)

modeled by TER94^A229E.UAS

(Liang et al., 2014)

modeled by TER94^R188Q.UAS

(Liang et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of P-stage, with Scer\GAL4^Act5C.PU

(Liang et al., 2014)

lethal - all die before end of P-stage, with Scer\GAL4^hs.PU

(Liang et al., 2014)

visible, with Scer\GAL4^GMR.PF

(Liang et al., 2014)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Liang et al., 2014)

mitochondrion, with Scer\GAL4^ninaE.PT

(Liang et al., 2014)

outer photoreceptor cell, with Scer\GAL4^ninaE.PT

(Liang et al., 2014)

Detailed Description

Statement

Reference

Expression of Der-1^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PF causes severe eye defects.

The cytosol of outer photoreceptors expressing Der-1^Scer\UAS.cLa under the control of Scer\GAL4^ninaE.PT is often filled with ER-resembling tubular membranes. Mitochondria are less than half the size of controls. Some of these atypical mitochondria show poor membrane integrity and intracristal swelling. The mitochondria in the inner photoreceptors appear normal.

(Liang et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Der-1^UAS.cLa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by TER94^UAS.cCa, Scer\GAL4^GMR.PF

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by TER94^A229E.UAS, Scer\GAL4^GMR.PF

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Hsap\VCP^UAS.cCa, Scer\GAL4^GMR.PF

(Liang et al., 2014)

Suppressor of

Statement

Reference

Der-1^UAS.cLa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, TER94^R188Q.UAS

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, TER94^A229E.UAS

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, TER94^R152H.UAS

(Liang et al., 2014)

Other

Statement

Reference

Der-1^UAS.cLa, Scer\GAL4^GMR.PF, TER94[+]/TER94^k15502 has lethal phenotype

(Liang et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Der-1^UAS.cLa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by TER94^UAS.cCa, Scer\GAL4^GMR.PF

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by TER94^A229E.UAS, Scer\GAL4^GMR.PF

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF has photoreceptor phenotype, suppressible by Decay^RNAi.UAS, Scer\GAL4^GMR.PF

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF has photoreceptor phenotype, suppressible by Dronc^RNAi.UAS.cOa, Scer\GAL4^GMR.PF

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Hsap\VCP^UAS.cCa, Scer\GAL4^GMR.PF

(Liang et al., 2014)

NOT suppressed by

Statement

Reference

Der-1^UAS.cLa, Scer\GAL4^GMR.PF has photoreceptor phenotype, non-suppressible by Drice^RNAi.UAS.cMa, Scer\GAL4^GMR.PF

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF has photoreceptor phenotype, non-suppressible by Dcp-1^RNAi.UAS, Scer\GAL4^GMR.PF

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF has photoreceptor phenotype, non-suppressible by Strica^RNAi.UAS, Scer\GAL4^GMR.PF

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF has photoreceptor phenotype, non-suppressible by Dredd^RNAi.UAS, Scer\GAL4^GMR.PF

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF has photoreceptor phenotype, non-suppressible by Damm^f02209, Scer\GAL4^GMR.PF

(Liang et al., 2014)

Suppressor of

Statement

Reference

Der-1^UAS.cLa, Scer\GAL4^GMR.PF is a suppressor of photoreceptor phenotype of Scer\GAL4^GMR.PF, TER94^R152H.UAS

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, TER94^R188Q.UAS

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF is a suppressor of photoreceptor phenotype of Scer\GAL4^GMR.PF, TER94^R188Q.UAS

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, TER94^A229E.UAS

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF is a suppressor of photoreceptor phenotype of Scer\GAL4^GMR.PF, TER94^A229E.UAS

(Liang et al., 2014)

Der-1^UAS.cLa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, TER94^R152H.UAS

(Liang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Der-1^Scer\UAS.cLa completely suppresses the rough eye phenotype and underlying photoreceptor degeneration seen when TER94^{A229E.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Der-1^Scer\UAS.cLa completely suppresses the rough eye phenotype and underlying photoreceptor degeneration seen when TER94^{R152H.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Der-1^Scer\UAS.cLa restores the reduction in cellular ATP level seen when TER94^{A229E.Scer\UAS} is expressed under the control of Scer\GAL4^hs.PU. The increase in ATPase activity is also rescued.

Expression of Der-1^Scer\UAS.cLa completely suppresses the rough eye phenotype and underlying photoreceptor degeneration seen when TER94^{R188Q.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of sip3^GD3145 does not reverse the suppressive effect of Der-1^Scer\UAS.cLa on the rough eye and photoreceptor degeneration phenotypes seen when TER94^{R188Q.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Ufd1-like^KK108134 does not reverse the suppressive effect of Der-1^Scer\UAS.cLa on the rough eye and photoreceptor degeneration phenotypes seen when TER94^{R188Q.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

Co-expression of TER94^Scer\UAS.cCa and Der-1^Scer\UAS.cLa results in a reduction in ATPase activity compared to controls.

Expression of TER94^Scer\UAS.cCa suppresses the eye phenotypes seen when Der-1^Scer\UAS.cLa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of TER94^{A229E.Scer\UAS} completely suppresses the severe eye defects seen when Der-1^Scer\UAS.cLa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Der-1^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PF in a heterozygous TER94^k15502 mutant background results in lethality.

Expression of Drice^{miRNA.Scer\UAS} fails to suppress the photoreceptor degeneration seen when Der-1^Scer\UAS.cLa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Dcp-1^{miRNA.Scer\UAS} fails to suppress the photoreceptor degeneration seen when Der-1^Scer\UAS.cLa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Strica^{miRNA.Scer\UAS} fails to suppress the photoreceptor degeneration seen when Der-1^Scer\UAS.cLa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Dredd^{miRNA.Scer\UAS} fails to suppress the photoreceptor degeneration seen when Der-1^Scer\UAS.cLa is expressed under the control of Scer\GAL4^GMR.PF.

Damm^f02209 fails to suppress the photoreceptor degeneration seen when Der-1^Scer\UAS.cLa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Decay^{miRNA.Scer\UAS} suppresses the photoreceptor degeneration seen when Der-1^Scer\UAS.cLa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Dronc^{miRNA.Scer\UAS} suppresses the photoreceptor degeneration seen when Der-1^Scer\UAS.cLa is expressed under the control of Scer\GAL4^GMR.PF.

(Liang et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\VCP^Scer\UAS.cCa suppresses the severe eye defects seen when Der-1^Scer\UAS.cLa is expressed under the control of Scer\GAL4^GMR.PF.

(Liang et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Der-1^Scer\UAS.cLa

Der-1^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Liang

Secondary FlyBase IDs

References (2)

Report Sections

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