Fecundity of homozygous females is strongly reduced. Despite normal morphology, <5% of deposited homozygous-null mutant embryos hatch into L1 larvae. Adult escapers are observed, but female fecundity and viability/development of the progeny are compromised. Females exhibit a prolonged egg retention phenotype, with the majority of embryos deposited after extended development inside their mothers' ovaries. A large fraction of homozygous embryos from 0-4 h AED remain in very early stages of development - in these, apposed male and female pronuclei, of which only one is undergoing mitosis while the other remains condensed, are frequently observed. Early mutant embryos also exhibit chromosomal abnormalities, including anaphase bridges and chromosome breaks.

P32⁴/P32² has partially lethal - majority die phenotype, enhanceable by Df(3R)Nlp-Nph