FlyBase Allele Report: Zzzz\poly-PR[PO-36.UAS]

General Information

Symbol

Zzzz\poly-PR^PO-36.UAS

Species

a. artificial

Name

FlyBase ID

FBal0300906

Feature type

allele

Associated gene

Zzzz\poly-PR

Associated Insertion(s)

Carried in Construct

P{UAS-poly-PR.PO-36}

Also Known As

UAS-PR36, PR36, PR-36

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Mizielinska et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-poly-PR.PO-36}

Cytology

Description

UAS regulatory sequences drive expression of 36 pure repeats of a "protein-only" version of Zzzz\poly-PR using alternative codons to those found within the GGGGCC repeat.

(Mizielinska et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Zzzz\poly-PR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis

CEA

(Hautbergue et al., 2017)

model of C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

CEA

(Rossi et al., 2023, Xu et al., 2023, Farrugia et al., 2022, Fumagalli et al., 2021, Kim et al., 2021, Park et al., 2021, Marmor-Kollet et al., 2020, Park et al., 2020, Ramesh et al., 2020, Xu and Xu, 2018)

CEC

(Kim et al., 2019, Xu et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of amyotrophic lateral sclerosis

is NOT ameliorated by SF2^GD12097

(Hautbergue et al., 2017)

model of C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

is ameliorated by lwr^UAS.cAa

(Marmor-Kollet et al., 2020)

is ameliorated by Upf1^UASp.GFP

(Xu et al., 2019)

is exacerbated by Mhc^KK102402

(Kim et al., 2019)

is exacerbated by Mhc^HMS01471

(Kim et al., 2019)

is exacerbated by Mhc^JF02069

(Kim et al., 2019)

is exacerbated by CG11414^HMC06381

(Park et al., 2021)

is exacerbated by CG11414^HMJ23120

(Park et al., 2021)

is exacerbated by CG14207^NIG.14207R

(Park et al., 2021)

is exacerbated by Capr^NIG.18811R

(Park et al., 2021)

is exacerbated by Clbn^GD7403

(Park et al., 2021)

is exacerbated by Edc3^NIG.6311R

(Park et al., 2021)

is exacerbated by Rack1^GL00637

(Park et al., 2021)

is exacerbated by Rack1^NIG.7111R

(Park et al., 2021)

is exacerbated by PAN3^NIG.11486R

(Park et al., 2021)

is exacerbated by PpV^NIG.12217R

(Park et al., 2021)

is exacerbated by Smg6^NIG.6369R

(Park et al., 2021)

is exacerbated by TER94^JF03402

(Park et al., 2021)

is exacerbated by Upf2^NIG.2253R

(Park et al., 2021)

is exacerbated by Upf3^NIG.11184R

(Park et al., 2021)

is exacerbated by Usp30^NIG.3016R

(Park et al., 2021)

is exacerbated by cup^NIG.11181R

(Park et al., 2021)

is exacerbated by eIF2β^NIG.4153R

(Park et al., 2021)

is exacerbated by eRF1^NIG.5605R

(Park et al., 2021)

is exacerbated by pico^NIG.11940R

(Park et al., 2021)

is exacerbated by pix^JF01190

(Park et al., 2021)

is exacerbated by rig^NIG.11171R

(Park et al., 2021)

is exacerbated by tral^NIG.10686R

(Park et al., 2021)

is exacerbated by stau^UAS.GFP

(Kim et al., 2021)

is exacerbated by Fib^KK108001

(Kim et al., 2021)

is exacerbated by stau^{UAS.Tag:NLS(Unk),Tag:HA}

(Kim et al., 2021)

is ameliorated by TBPH^UAS.cUa

(Kim et al., 2021)

is ameliorated by bumpel^{UAS.ORF.GW.Tag:HA}

(Xu et al., 2023)

is exacerbated by ArfGAP1^HMJ30212

(Rossi et al., 2023)

is exacerbated by ergic53^HMC03809

(Rossi et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 2 )

FlyBase curator comment: "FTDALS1" is associated with G4C2 transcripts and dipeptide repeat proteins derived from human C9ORF72.

(Kim et al., 2019)

FlyBase curator comment: "FTDALS1" is associated with dipeptide repeat proteins derived from human C9ORF72.

(Xu et al., 2019)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color | adult stage, with Scer\GAL4^GMR.PF

(Ramesh et al., 2020)

abnormal locomotor behavior | adult stage, with Scer\GAL4^da.G32

(Hautbergue et al., 2017)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155

(Xu et al., 2019)

abnormal locomotor behavior | adult stage, with Scer\GAL4^Toll-6-D42

(Baldwin et al., 2016)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Baldwin et al., 2016)

decreased cell number | adult stage, with Scer\GAL4^Toll-6-D42

(Xu et al., 2019)

decreased size | adult stage, with Scer\GAL4^GMR.PU

(Morón-Oset et al., 2023)

increased cell death | adult stage, with Scer\GAL4^GMR.PF

(Ramesh et al., 2020)

partially lethal | heat sensitive, with Scer\GAL4^GMR.PU

(Mizielinska et al., 2014)

partially lethal - majority live, with Scer\GAL4^GMR.PU

(Morón-Oset et al., 2023)

short lived, with Scer\GAL4^elav-C155

(Xu et al., 2019)

short lived | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023, Xu et al., 2023)

visible, with Scer\GAL4^GMR.PU

(Mizielinska et al., 2014)

visible | adult stage, with Scer\GAL4^GMR.PF

(Ramesh et al., 2020, Hautbergue et al., 2017)

visible | adult stage, with Scer\GAL4^GMR.PU

(Morón-Oset et al., 2023, Park et al., 2021, Marmor-Kollet et al., 2020)

Phenotype Manifest In

adult antennal lobe, with Scer\GAL4^Toll-6-D42

(Xu et al., 2019)

adult motor neuron, with Scer\GAL4^Toll-6-D42

(Xu et al., 2019)

embryonic/larval motor neuron | third instar larval stage, with Scer\GAL4^CCAP.PU

(Baldwin et al., 2016)

eye, with Scer\GAL4^GMR.PF

(Ramesh et al., 2020)

eye, with Scer\GAL4^GMR.PU

(Morón-Oset et al., 2023, Park et al., 2021, Marmor-Kollet et al., 2020, Mizielinska et al., 2014)

eye | adult stage, with Scer\GAL4^GMR.PF

(Hautbergue et al., 2017)

Detailed Description

Statement

Reference

The expression of Zzzz\poly-PR^PO-36.UAS under the control of Scer\GAL4^GMR.PU induces a eye degeneration phenotype.

(Park et al., 2021)

Expression of Zzzz\poly-PR^PO-36.UAS under the control of Scer\GAL4^GMR.PU leads to presence of black necrotic tissue in the eye.

(Marmor-Kollet et al., 2020)

The expression of Zzzz\poly-PR^PO-36.UAS under the control of Scer\GAL4^GMR.PF external eye degeneration including necrotic patches.

(Ramesh et al., 2020)

Adults expressing Zzzz\poly-PR^PO-36.UAS under the control of Scer\GAL4^Toll-6-D42 show loss of motor neurons in the antennal lobe. Adults expressing Zzzz\poly-GR^36.UAS.EGFP under the control of Scer\GAL4^elav-C155 also show a significant decrease in climbing ability, as compared to controls.

(Xu et al., 2019)

The expression of Zzzz\poly-GR^{PO-36.Scer\UAS} leads to a severe rough eye phenotype (Scer\GAL4^GMR.PF-driven expression) and to a moderate decrease in adult locomotor activity in climbing assays (Scer\GAL4^da.G32-driven expression), as compared to controls.

(Hautbergue et al., 2017)

The expression of Zzzz\poly-PR^PO-36.UAS under the control of Scer\GAL4^CCAP.PI leads a significant decrease in the axonal transport of both mitochondria and (NPY-positive) vesicles (i.e. significant decreases in anterograde and retrograde transport and reciprocal significant increase in the stationary fraction), in third instar larval motor neurons, as compared to controls.

Expression under the control of Scer\GAL4^Toll-6-D42 leads to a significant decrease in larval and adult locomotion (in climbing and crawling assays, respectively), as compared to controls.

(Baldwin et al., 2016)

Expression of Zzzz\poly-PR^{PO-36.Scer\UAS} using Scer\GAL4^GMR.PU causes eye degeneration and lethality.

(Mizielinska et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS has visible | adult stage phenotype, enhanceable by CG11414^HMC06381, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS has visible | adult stage phenotype, enhanceable by Clbn^HMJ23825, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS has visible | adult stage phenotype, enhanceable by Ltn1^G9156, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS has visible | adult stage phenotype, enhanceable by CG11414^HMJ23120, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS has visible | adult stage phenotype, enhanceable by Nulp1^G6757, Scer\GAL4^GMR.PU

(Park et al., 2021)

Suppressed by

Statement

Reference

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-PR^PO-36.UAS has short lived | RU486 conditional phenotype, suppressible by Scer\GAL4^{elav.Switch.PO}/bumpel^{UAS.ORF.GW.Tag:HA}

(Xu et al., 2023)

Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS has visible | adult stage phenotype, suppressible by lwr^UAS.cAa/Scer\GAL4^GMR.PU

(Marmor-Kollet et al., 2020)

NOT suppressed by

Statement

Reference

Scer\GAL4^da.G32, Zzzz\poly-PR^PO-36.UAS has abnormal locomotor behavior | adult stage phenotype, non-suppressible by SF2^GD12097, Scer\GAL4^da.G32

(Hautbergue et al., 2017)

Scer\GAL4^GMR.PF, Zzzz\poly-PR^PO-36.UAS has visible | adult stage phenotype, non-suppressible by SF2^GD12097, Scer\GAL4^GMR.PF

(Hautbergue et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS has eye phenotype, enhanceable by CG11414^HMC06381, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS has eye phenotype, enhanceable by Clbn^HMJ23825, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS has eye phenotype, enhanceable by Ltn1^G9156, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS has eye phenotype, enhanceable by CG11414^HMJ23120, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS has eye phenotype, enhanceable by Nulp1^G6757, Scer\GAL4^GMR.PU

(Park et al., 2021)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS has eye phenotype, suppressible by lwr^UAS.cAa/Scer\GAL4^GMR.PU

(Marmor-Kollet et al., 2020)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\poly-PR^PO-36.UAS has eye | adult stage phenotype, non-suppressible by SF2^GD12097, Scer\GAL4^GMR.PF

(Hautbergue et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The severe rough eye phenotype and the moderate decrease in adult locomotor induced by the expression of Zzzz\poly-PR^{PO-36.Scer\UAS}, under the control of Scer\GAL4^GMR.PF and Scer\GAL4^da.G32, respectively, are not suppressed by the co-expression of SF2^GD12097.

(Hautbergue et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58694

w¹¹¹⁸; P{UAS-poly-PR.PO-36}attP40

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

PR-36

(Baldwin et al., 2016)

Zzzz\poly-PR^{PO-36.Scer\UAS}

Zzzz\poly-PR^PO-36.UAS

Name Synonyms

Secondary FlyBase IDs

References (19)

Report Sections

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