FlyBase Allele Report: Dmel\lwr[UAS.cAa]

General Information

Symbol

Dmel\lwr^UAS.cAa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Apionishev

FlyBase ID

FBal0122955

Feature type

allele

Associated gene

Dmel\lwr

Associated Insertion(s)

Carried in Construct

P{UAS-lwr.A}

Also Known As

UAS-lwr, UAS-Ubc9^WT

Key Links

Transgenic product class

wild_type

(Apionishev et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Apionishev et al., 2001)

Mutagen

in vitro construct

(Apionishev et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-lwr.A}

Cytology

Description

UASt regulatory sequences drive expression of the lwr coding region.

(Apionishev et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

lwr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\poly-PR^PO-36.UAS

(Marmor-Kollet et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of lwr^Scer\UAS.cAa, under the control of Scer\GAL4^lz-gal4, does not lead to a significant change in crystal cell number.

(Chiu et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

lwr^UAS.cAa/Scer\GAL4^GMR.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS

(Marmor-Kollet et al., 2020)

Scer\GAL4^{VP16.nanos.UTR}/lwr^UAS.cAa is a suppressor | partially of abnormal cell migration phenotype of Scer\GAL4^{VP16.nanos.UTR}, dpp^UAS.cSa

(Deshpande et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

lwr^UAS.cAa/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\poly-PR^PO-36.UAS

(Marmor-Kollet et al., 2020)

Scer\GAL4^{VP16.nanos.UTR}/lwr^UAS.cAa is a suppressor | partially of germline cell phenotype of Scer\GAL4^{VP16.nanos.UTR}, dpp^UAS.cSa

(Deshpande et al., 2014)

Scer\GAL4^{VP16.nanos.UTR}/lwr^UAS.cAa is a suppressor | partially of gonad phenotype of Scer\GAL4^{VP16.nanos.UTR}, dpp^UAS.cSa

(Deshpande et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of lwr^Scer\UAS.cAa with dpp^Scer\UAS.cSa in primordial germ cells, under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not restore the number of primoridal germ cells to wild-type, but does alter the distribution of these cells to a more wild-type manner.

(Deshpande et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

lwr^UAS.cAa/Scer\GAL4^GH146 rescues lwr¹³

(Berdnik et al., 2012)

Scer\GAL4^76B/lwr^UAS.cAa rescues lwr⁵/lwr^4-3

(Paddibhatla et al., 2010)

Partially rescues

lwr^UAS.cAa/Scer\GAL4^Cg.PA partially rescues lwr⁵/lwr^4-3

(Chiu et al., 2005)

Scer\GAL4^nanos.PG/lwr^UAS.cAa partially rescues lwr⁵/lwr^4-3

(Apionishev et al., 2001)

lwr^UAS.cAa/Scer\GAL4^e22c partially rescues lwr⁵/lwr^4-3

(Apionishev et al., 2001)

Fails to rescue

lwr^UAS.cAa/Scer\GAL4^e22c fails to rescue lwr⁵

(Apionishev et al., 2001)

Scer\GAL4^nanos.PG/lwr^UAS.cAa fails to rescue lwr⁵

(Apionishev et al., 2001)

Comments

Expression of lwr^Scer\UAS.cAa under the control of Scer\GAL4^GH146 fully rescues the dendrite mistargeting defects in lwr¹³ mutants.

(Berdnik et al., 2012)

Expression of lwr^Scer\UAS.cAa in a subset of the lymph gland cells under the control of Scer\GAL4^76B significantly reduced the infiltration index in lwr^4-3/lwr⁵ mutants, indicating an anti-inflammatory role for lwr.

(Paddibhatla et al., 2010)

Expression of lwr^Scer\UAS.cAa under the control of Scer\GAL4^Cg.PA partially rescues the developmental delay of lwr^4-3/lwr⁵ mutants, increasing the amount of animals undergoing pupariation from 25 to 61% at day 8. However, there is no clear rescue of the melanotic tumour phenotype.

(Chiu et al., 2005)

lwr^Scer\UAS.cAa rescues lwr^4-3/lwr⁵ females to adulthood when expressed using Scer\GAL4^e22c or Scer\GAL4^nos.PG, however the rescued flies are sterile. The ovaries develop to stage 8 or 9 (Scer\GAL4^nos.PG) or stage 2 (Scer\GAL4^e22c).