FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Nlg3KO88
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General Information
Symbol
Dmel\Nlg3KO88
Species
D. melanogaster
Name
FlyBase ID
FBal0301058
Feature type
allele
Associated gene
Dmel\Nlg3
Associated Insertion(s)
TI{TI}Nlg3KO88
Carried in Construct
Also Known As
dnlg3KO88
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Xing et al., 2014)
Mutagen
ends-out gene targeting
(Xing et al., 2014)
Progenitor genotype
Associated Insertion(s)
TI{TI}Nlg3KO88
Cytology
Description

3849bp of the Nlg3 genomic region (nucleotides -1556 to +2293) have been replaced by a loxP cassette containing a w+mW.hs marker.

(Xing et al., 2014)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
3R:7,601,456..7,605,303 [-]
Comment:

Nlg3 genomic region (nucleotides -1556 to +2293, boundaries inferred from primer sequences) have been replaced by a P1\loxP cassette containing a w[+mW.hs] marker.

(Xing et al., 2014)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Nlg3KO88/Nlg3KO88 and Nlg3KO127/Nlg3KO88 mutant adults show decreased daily locomotor activity, as compared to controls.

      (Wu et al., 2018)

      Excitatory junctional potential (EJP) amplitude and miniature excitatory junctional potential (mEJP) frequency are significantly reduced at the Nlg3KO88/Nlg3f00777 third larval instar neuromuscular junction compared to wild type. mEJP amplitude is not significantly affected.

      Nlg3KO88/Nlg3f00777 third larval instar larvae show a significant increase in bouton number and a significant decrease in bouton size at the neuromuscular junction compared to wild type. Synaptic branch number at the neuromuscular junction is not significantly affected.

      Ultrastructurally, the neuromuscular junction of Nlg3KO88/Nlg3f00777 larvae shows a number of defects compared to wild type. There is a shift in the distribution of synaptic vesicle diameter to larger values and the number of large vesicles greater than 80nm in diameter is dramatically increased. The number of vesicles at the active zone is significantly decreased, and the postsynaptic density length is significantly increased.

      Synaptic endocytosis at the neuromuscular junction is impaired in Nlg3KO88/Nlg3f00777 larvae.

      (Xing et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Nlg3KO88/Nlg3KO127 is rescued by Nlg3UAS.cXa/Scer\GAL4Mhc.PU

      (Xing et al., 2014)
      Comments

      Muscle-specific expression of Nlg3Scer\UAS.cXa (under the control of Scer\GAL4Mhc.PU) rescues the defects in bouton number and size seen at the neuromuscular junction in Nlg3KO88/Nlg3KO127 larvae. Neuron-specific expression (under the control of Scer\GAL4elav.PU) does not rescue this phenotype.

      (Xing et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Nlg3KO88
      dnlg3KO88
      (Wu et al., 2018, Xing et al., 2014)
      nlg3KO88
      (Guangming et al., 2023)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)