FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Nlg3UAS.cXa
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General Information
Symbol
Dmel\Nlg3UAS.cXa
Species
D. melanogaster
Name
FlyBase ID
FBal0301060
Feature type
allele
Associated gene
Dmel\Nlg3
Associated Insertion(s)
Carried in Construct
P{UAS-Nlg3.X}
Key Links
Transgenic product class
wild_type
(Xing et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Xing et al., 2014)
Mutagen
in vitro construct
(Xing et al., 2014)
Progenitor genotype
Carried in construct
P{UAS-Nlg3.X}
Cytology
Description

UASt regulatory sequences drive expression of the full-length Nlg3 coding sequence.

(Xing et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Nlg3
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Nlg3UAS.cXa under the control of Scer\GAL4how-24B induces a severe decrease in the number of boutons at the third instar larval neuromuscular junction, as compared to controls.

      The expression of Nlg3UAS.cXa under the control of either Scer\GAL4elav.PU or Scer\GAL4how-24B does not induce adult locomotor activity defects.

      The expression of either Nlg3Δ10.UAS or Nlg3UAS.cXa under the control of Scer\GAL4how-24B rescues the increased bouton number in the third instar larval neuromuscular junction of Nlg3KO127, even leading to fewer boutons compared to controls.

      (Wu et al., 2018)

      Muscle-specific expression of Nlg3Scer\UAS.cXa under the control of Scer\GAL4Mhc.PU results in a significant decrease in bouton number and a significant increase in bouton size at the third larval instar neuromuscular junction. Neuron-specific expression (under the control of Scer\GAL4elav.PU) has no significant effect on bouton number or size.

      Expression of two copies of Nlg3Scer\UAS.cXa under the control of two copies of Scer\GAL4how-24B results in larval lethality. Expression of Nlg3Scer\UAS.cXa under the control of one copy of Scer\GAL4how-24B results in a significant decrease in bouton number at the third larval instar neuromuscular junction.

      (Xing et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Nlg3UAS.cXa/Scer\GAL4elav.PU rescues Nlg3KO127

      (Wu et al., 2018)

      Nlg3UAS.cXa/Scer\GAL4Mhc.PU rescues Nlg3KO88/Nlg3KO127

      (Xing et al., 2014)
      Fails to rescue

      Nlg3UAS.cXa/Scer\GAL4how-24B fails to rescue Nlg3KO127

      (Wu et al., 2018)
      Comments

      Muscle-specific expression of Nlg3Scer\UAS.cXa (under the control of Scer\GAL4Mhc.PU) rescues the defects in bouton number and size seen at the neuromuscular junction in Nlg3KO88/Nlg3KO127 larvae. Neuron-specific expression (under the control of Scer\GAL4elav.PU) does not rescue this phenotype.

      (Xing et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Nlg3Scer\UAS.cXa
      Nlg3UAS.cXa
      Name Synonyms
      Secondary FlyBase IDs
        References (2)