FB2026_02 , released June 18, 2026
Allele: Dmel\dor35
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General Information
Symbol
Dmel\dor35
Species
D. melanogaster
Name
FlyBase ID
FBal0301188
Feature type
allele
Associated gene
Dmel\dor
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Fernandes et al., 2014)
Progenitor genotype
Cytology
Description

Splice donor mutation that results in a protein with 56 additional amino acids and an early stop codon.

(Fernandes et al., 2014)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:1,667,613..1,667,613 [+]
Nucleotide change:

G1667613A

Comment:

G to A mutation in splice donor site.

(Fernandes et al., 2014)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  neurodegenerative disease
      modeled by sky2
      (Fernandes et al., 2014)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Females in which the eye is homozygous show electroretinogram defects.

      The mean amplitude of the excitatory junctional current at the neuromuscular junction is not significantly different from that of controls in mutant larvae. However, the mean amplitude of the spontaneous miniature excitatory junctional current is significantly increased and quantal content is significantly decreased. FM 1-43 dye labelling indicates that synaptic vesicle recycling is reduced during stimulation. The readily releasable vesicle pool (RRP) is approximately 30% smaller in the mutant larvae compared to controls.

      (Fernandes et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      dor[+]/dor35 is a suppressor | partially of abnormal neurophysiology | somatic clone | adult stage phenotype of sky2

      (Fernandes et al., 2014)

      dor[+]/dor35 is a suppressor of abnormal neuroanatomy | somatic clone | progressive phenotype of sky2

      (Fernandes et al., 2014)

      dor[+]/dor35 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of sky1/sky2

      (Fernandes et al., 2014)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      dor[+]/dor35 is a suppressor of retina | somatic clone | progressive phenotype of sky2

      (Fernandes et al., 2014)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The electroretinogram defects seen in homozygous sky2 eyes are partially suppressed if the flies are also heterozygous for dor35.

      The progressive retina neurodegeneration seen in flies containing homozygous sky2 eyes is suppressed if the flies are also heterozygous for dor35.

      The increase in excitatory junctional current amplitude at the neuromuscular junction which is seen in sky1/sky2 larvae is suppressed if they are also heterozygous for dor35.

      The increased synaptic vesicle trafficking to endosomes seen at the neuromuscular junction in sky1/sky2 larvae is not suppressed if the larvae are also heterozygous for dor35.

      (Fernandes et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      dor35 is rescued by dor1-1002.UAS/Scer\GAL4da.PU

      (Fernandes et al., 2014)
      Comments

      The increase in miniature excitatory junctional current amplitude and reduction in quantal content which is seen at the neuromuscular junction in dor35 larvae is rescued by expression of dor1-1002.Scer\UAS under the control of Scer\GAL4da.PU. The reduction in synaptic vesicle recycling is at least partially rescued.

      Expression of dor1-1002.Scer\UAS under the control of Scer\GAL4VGlut.PU does not rescue the increase in miniature excitatory junctional current amplitude which is seen at the neuromuscular junction in dor35 larvae, while the amplitude of the evoked excitatory junctional current is significantly increased compared to the single mutant larvae. The reduction in synaptic vesicle recycling is at least partially rescued.

      (Fernandes et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      dor35
      (Fernandes et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)